Identification of key parameters in adjuvant efficiency

疫苗产品制剂的生产在其商业化之前需要大量投资。在这些努力中的投入为生产这些产品的公司带来了投资回报，并对人口的健康和寿命产生了重大影响。目前生产的大多数疫苗都针对一种以上的病毒。这种疫苗的配方很复杂，还包括添加佐剂，这些佐剂被证明可以增强我们对特定疫苗药物的反应。在这种情况下，一些疫苗没有显示出预期的免疫应答，这可能与抗原在佐剂上的吸附程度有关，这与Avaccine免疫原性有关。在这个项目中，我们感兴趣的是佐剂形态学和表面化学的表征，并将其与吸附抗原的能力联系起来。这些信息将允许评估佐剂表面的重现性，并探索调整其表面化学的方法，以确保最佳的佐剂性能。在这项工作中，我们将使用X射线光电子能谱，拉曼和FTIR光谱，以及扫描电子显微镜，以了解在不同条件下的佐剂表面的状态。将使用各种活化过程以及化学处理来修饰佐剂表面并监测其对选定抗原的吸附特性。最终目标是开发一种方法，在疫苗配方中使用之前对生产的佐剂进行相关的质量控制。

Production of vaccine product formulation requires large investments before their commercialization. Successin these endeavours results in investment returns for the companies producing them and large impact onpopulation health and longevity. Most vaccine produced today are targeting more than one virus. Theformulation is complex and involves the addition of adjuvants that are substances that are shown to boost ourresponse to a given vaccine drug. In this context, some vaccines do not display the expected immune response.This maybe linked to the extend of antigens adsorption on the adjuvant, which was shown to be linked to avaccine immunogenicity. In this project we are interested in the characterization of the adjuvant morphologyand surface chemistry and to link this to its ability to adsorbed antigens. This information would allow to assesshow reproducible is the adjuvant surface and to explore ways to tailor its surface chemistry to ensure optimumadjuvant performances. In this work we will use x-ray photoelectron spectroscopy, Raman and FTIRspectroscopy, as well as scanning electron microscopy to understand the state of the adjuvant surface underdifferent conditions. Various activation process as well as chemical treatments will be use to modify theadjuvant surface and monitor its adsorption properties toward selected antigens. The ultimate goal is to developa methodology to perform relevant quality control on the produced adjuvant prior to its use in vaccineformulation.