Identification of ammonia oxidizing bacteria and archaea within industrial wastewater treatment system bioreactors

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Although ammonia oxidation is an important process for bioreactors treating industrial waste, the identities andfunctional contributions of the microorganisms catalyzing this important process remains unknown for manyengineered systems. This research partnership will test samples taken temporally from a lab-scale pilotbioreactor to identify the active ammonia oxidizers and assess the ability of genes collected from these systemsto identify the functions of detected microorganisms. This research is important because bioreactor systemstreating industrial waste are notoriously unstable and Tetra Tech is currently unable to identify ammoniaoxidizers upon which their system performance depends. Identifying microorganisms involved in ammoniaoxidation will help identify the causes of instability, possible solutions related to the manipulation of processparameters, and a protocol that Tetra Tech can follow in the future for monitoring bioreactor microbialcommunities.