Identification of ammonia oxidizing bacteria and archaea within industrial wastewater treatment system bioreactors

虽然氨氧化是生物反应器处理工业废水的一个重要过程，但催化这一重要过程的微生物的特性和功能贡献在许多工程系统中仍然未知。该研究伙伴关系将测试从实验室规模的中试生物反应器临时采集的样品，以识别活性氨氧化剂，并评估从这些系统收集的基因识别被检测微生物功能的能力。这项研究很重要，因为处理工业废物的生物反应器系统是出了名的不稳定，而利乐科技目前无法确定其系统性能所依赖的氨氧化剂。确定参与氨氧化的微生物将有助于确定不稳定的原因，与操作工艺参数相关的可能解决方案，以及利乐科技未来可以遵循的监测生物反应器微生物群落的协议。

Although ammonia oxidation is an important process for bioreactors treating industrial waste, the identities andfunctional contributions of the microorganisms catalyzing this important process remains unknown for manyengineered systems. This research partnership will test samples taken temporally from a lab-scale pilotbioreactor to identify the active ammonia oxidizers and assess the ability of genes collected from these systemsto identify the functions of detected microorganisms. This research is important because bioreactor systemstreating industrial waste are notoriously unstable and Tetra Tech is currently unable to identify ammoniaoxidizers upon which their system performance depends. Identifying microorganisms involved in ammoniaoxidation will help identify the causes of instability, possible solutions related to the manipulation of processparameters, and a protocol that Tetra Tech can follow in the future for monitoring bioreactor microbialcommunities.