Validation of ammonia-oxidizing archaea and comammox Nitrospira for commercial application

氨氧化古菌和comammox Nitrospira 商业应用的验证

• 批准号: 576114-2022
• 负责人: Neufeld, JoshJD
• 金额: $ 1.46万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=745406
• 关键词: Validation; ammonia; oxidizing; archaea; comammox

Nitrification (i.e., ammonia oxidation to nitrate via nitrite) is an important microbial process within natural and engineered systems. Waste degradation and the metabolism of aquatic species produces ammonia, and this nitrogenous compound is toxic to aquatic life unless treated. In an established aquarium biofilter, nitrifying microorganisms oxidize ammonia to the less-toxic end product nitrate. Newly established aquaria are often supplemented with ammonia-oxidizing bacteria (AOB) associated with the product "Cycle" from Hagen Industries to help establish nitrification and prevent toxicity and associated fish disease and death. Although aerobic nitrification was historically thought to be divided between AOB and nitrite-oxidizing bacteria (NOB), ammonia-oxidizing archaea (AOA) and complete ammonia oxidizing (comammox) Nitrospira, capable of fully oxidizing ammonia to nitrate via nitrite, have been recently discovered. Data from our laboratory show that AOA and comammox Nitrospira dominate aquarium biofilters yet are absent from Cycle. This proposed NSERC Alliance project will investigate the nitrifying capabilities of AOA and comammox Nitrospira within the context of freshwater aquaria, and will generate data useful to Hagen Industries for development of an improved Cycle product. Existing AOA and comammox Nitrospira enrichment cultures will be further enriched and the nitrification activity of isolated and mixed cultures will be evaluated against the current Cycle product. A clinoptilolite ("zeolite") substrate will be investigated for its ability to act as a carrier for microbial biofilm containing AOA and comammox Nitrospira cells, with qPCR and microscopy used to quantify and visualize microbial growth. The long-term viability of AOA and comammox Nitrospira on a zeolite-containing medium will be evaluated under different growth and storage conditions to investigate potential temporal changes in activity due to storage. An improved Cycle prototype product will be evaluated within freshwater aquaria using microbial community profiling and water chemistry analyses. The discoveries made through this proposed work will directly support Canadians through collaboration with Hagen Industries and will further contribute to Canadian-based applied freshwater ecology and nitrification research.

硝化（即，通过亚硝酸盐氧化为硝酸盐氧化）是自然和工程系统中的重要微生物过程。废物降解和水生植物的代谢会产生氨，除非治疗，否则这种氮化合物对水生生物有毒。在已建立的水族馆生物滤池中，硝化微生物将氨们氧化为氧化较少的末端产物硝酸盐。新成立的水族馆通常补充了与哈根工业“周期”相关的氨氧化细菌（AOB），以帮助建立硝化和预防毒性，并相关的鱼类疾病和死亡。尽管历史上认为有氧硝化作用在AOB和亚硝酸盐氧化细菌（NOB），氨氧化古细菌（AOA）和完全氧化氨（Comammox）硝酸螺旋体之间，能够通过硝酸盐通过硝酸盐进行全硝酸盐的氧化氧化（Comammox）硝酸盐，最近发现了。我们实验室的数据表明，AOA和COMAMMOX硝基螺旋体占主导地位的水族馆生物滤器，但循环中没有。该拟议的NSERC联盟项目将在淡水水族馆的背景下调查AOA和Comammox硝基螺旋体的硝化能力，并将生成对Hagen Industries有用的数据，以开发改进的周期产品。现有的AOA和COMAMMOX硝化含量富集培养物将进一步富集，并将针对当前周期产品评估分离和混合培养物的硝化活性。将研究一个粘钙化石（“沸石”）底物，其能够充当含有AOA和Comammox硝基螺旋拉细胞的微生物生物膜的载体，其QPCR和显微镜用于量化和可视化微生物生长。在不同的生长和存储条件下，将评估AOA和Comammox硝基螺旋体在含沸石培养基上的长期生存能力，以研究由于储存而导致的活动的潜在时间变化。改进的循环原型产品将在淡水水族箱中使用微生物群落分析和水化学分析进行评估。通过这项拟议的工作发现的发现将通过与Hagen Industries的合作直接支持加拿大人，并将进一步为加拿大的应用淡水生态学和硝化研究做出贡献。