Value-added applications for industrial waste eggshell membrane for cosmetic application

大量的蛋壳废料是鸡蛋加工业的副产品。处理这些蛋壳会造成环境和经济负担，因此，这些材料的替代用途将具有明显的好处。蛋壳废弃物是一种重要的高附加值资源，可用于提取新型生物活性肽，用于人体健康、化妆品、生物医学和营养保健品等领域。提取有价值的蛋壳衍生成分的关键步骤是实现膜与富钙壳的清洁分离。虽然迄今为止的研究已经证明蛋壳膜衍生蛋白可能具有生物活性/营养潜力，但对蛋壳膜衍生肽，包括其生产和潜在生物活性知之甚少。目的：1）探索可工业化生产的蛋壳膜水解方法。2)研究从蛋壳膜废料中提取/生产新型肽并鉴定其特性;3）研究其在化妆品中的用途/应用。生物活性膜衍生肽的生产然后通过使用市售蛋白酶（包括胶原酶和碱性蛋白酶）的酶促水解来进行，以产生胶原衍生肽/生物活性肽。不同的酶和酶组合将被检查，以确定肽生产的最佳方法。蛋壳膜肽在化妆品应用中的潜在活性也将使用培养的皮肤细胞系（角质形成细胞和成纤维细胞）进行检查。体外实验观察蛋壳膜衍生肽对成纤维细胞在胶原凝胶上的增殖作用。采用体外细胞培养模型分析成纤维细胞迁移和酶活性（透明质酸酶、酪氨酸酶、弹性蛋白酶等）。加拿大鸡蛋产业为经济创造了超过10亿美元的收入，但它很容易受到消费者对鸡蛋态度和看法的变化的影响。因此，加拿大蛋品产业的重要性在于使蛋品利用多样化，超越传统的食品用途，并开发新的增值生物产品和保健产品。

A significant amount of eggshell waste is produced as a by-product of the egg processing industry. Disposal ofthese eggshells creates an environmental and financial burden and, therefore, alternative uses for thesematerials would be of obvious benefit. Eggshell waste may be an important value-added resource for theextraction of novel bioactive peptides for human health, cosmetic, biomedical and nutraceutical applications. Acritical step to the extraction of valuable eggshell-derived components is to achieve a clean separation of themembranes from the calcium-rich shell. While studies up until now have demonstrated that eggshellmembrane-derived proteins may have bioactive/nutraceutical potential, little is known about eggshellmembrane-derived peptides, including their production and potential bioactivity. The objectives: 1) To exploremethods of eggshell membrane hydrolyzation capable of industrial production. 2) To studyextraction/production and characterization of novel peptides from eggshell membrane waste and;3) To examinetheir use/applications in cosmetic products. Production of bioactive membrane-derived peptides will then becarried out by enzymatic hydrolysis using commercially available proteases, including collagenase andalcalase, to produce collagen-derived peptides/bioactive peptides. Different enzymes and enzyme combinationswill be examined to determine the best method of peptide production. The potential activity of eggshellmembrane peptides in cosmetic applications will also be examined using cultured skin cell lines (keratinocytesand fibroblasts). The proliferation of fibroblasts on the collagen gel by eggshell membrane derived peptideswill be assessed in vitro. Fibroblasts migration and enzyme activity (hyuaronase, tyrocinase, elastase etc) willbe analyzed using in vitro cell culture model. The Canadian egg industry generates over $1 billion into theeconomy, but it is vulnerable to shifts in consumer attitudes and perceptions about eggs. It is thereforeimportant for the Canadian egg industry to diversify egg utilizations beyond traditional food uses and developnew value-added bio-products and heath products.