Value-added applications for industrial waste eggshell membrane for cosmetic application

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A significant amount of eggshell waste is produced as a by-product of the egg processing industry. Disposal ofthese eggshells creates an environmental and financial burden and, therefore, alternative uses for thesematerials would be of obvious benefit. Eggshell waste may be an important value-added resource for theextraction of novel bioactive peptides for human health, cosmetic, biomedical and nutraceutical applications. Acritical step to the extraction of valuable eggshell-derived components is to achieve a clean separation of themembranes from the calcium-rich shell. While studies up until now have demonstrated that eggshellmembrane-derived proteins may have bioactive/nutraceutical potential, little is known about eggshellmembrane-derived peptides, including their production and potential bioactivity. The objectives: 1) To exploremethods of eggshell membrane hydrolyzation capable of industrial production. 2) To studyextraction/production and characterization of novel peptides from eggshell membrane waste and;3) To examinetheir use/applications in cosmetic products. Production of bioactive membrane-derived peptides will then becarried out by enzymatic hydrolysis using commercially available proteases, including collagenase andalcalase, to produce collagen-derived peptides/bioactive peptides. Different enzymes and enzyme combinationswill be examined to determine the best method of peptide production. The potential activity of eggshellmembrane peptides in cosmetic applications will also be examined using cultured skin cell lines (keratinocytesand fibroblasts). The proliferation of fibroblasts on the collagen gel by eggshell membrane derived peptideswill be assessed in vitro. Fibroblasts migration and enzyme activity (hyuaronase, tyrocinase, elastase etc) willbe analyzed using in vitro cell culture model. The Canadian egg industry generates over $1 billion into theeconomy, but it is vulnerable to shifts in consumer attitudes and perceptions about eggs. It is thereforeimportant for the Canadian egg industry to diversify egg utilizations beyond traditional food uses and developnew value-added bio-products and heath products.