Value-added applications of eggshell membrane-derived bioactive peptides for cosmetic application

加拿大每年生产约90亿个鸡蛋，其中约27%在鸡蛋破碎时加工，产生低价值的蛋壳作为副产品。加拿大家禽业每年产生约1.5吨蛋壳膜（ESM）废物。发现蛋壳废料的新的增值应用将具有重要的经济和环境效益。由于牛海绵状脑病（bovine spongiform encephalopathy，BSE）的严重危害，蛋壳胶原蛋白和胶原蛋白肽是一种很有吸引力的替代品，但其在重金属离子吸附基质、烧伤敷料、细胞培养基质和酶固定化平台等方面的应用较少。需要进一步的研究来验证ESM肽在特定化妆品应用中的生物活性。假设：ESM肽可以应用于化妆品，包括皮肤移植，刺激人类皮肤成纤维细胞的生长，保湿，美白和减少皮肤炎症的产生。目标：1）体外观察ESM多肽对成纤维细胞在胶原凝胶上增殖的影响。2）体外细胞培养模型观察成纤维细胞迁移及酶活性（透明质酸酶、酪氨酸酶、弹性蛋白酶等）。该项目的主要目标是1）促进成纤维细胞增殖，2）抗炎和3）美白。成纤维细胞迁移是保持皮肤弹性、水分的重要因素，我们测量了ESM肽对细胞增殖和透明质酸酶/酯酶活性的影响。将通过检测TNF-？测定抗炎活性，MCP-1、IL-6是人类皮肤中典型的炎症介质，与皮肤抗衰老有关。最后，通过酪氨酸酶活性和抗紫外线活性的测定，对ESM肽的美白活性进行了研究。该项目的最终目标是从工业废物中开发具有附加值的无害环境管理肽。ESM衍生的生物材料对世界市场具有吸引力，因为个人对猪或牛来源的材料有宗教担忧。

Canadian produces about 9 billion eggs per year and about 27% are processed at egg breaking which yield lowvalue eggshell as a by-product. The Canadian poultry industry produces about 1.5 tons eggshell membrane(ESM) waste annually. The discovery of novel value-added applications for eggshell waste would haveimportant financial and environmental benefits. Eggshell-derived collagen and collagen-derived peptides are anattractive replacement for bovine collagen due to the concern over bovine spongiform encephalopathy (BSE).Only a few applications have been reported, including matrices for adsorption of heavy metal ions, biologicaldressings for burns, substrates for cell culture and platforms for enzyme immobilization. Further studies arerequired to validate the biological activity of ESM peptides for specific cosmetic application. Hypothesis: ESMpeptides can be applied in cosmetics including skin grafts, stimulate the growth of human skin fibroblasts,moisture, whitening and reduce the production of inflammation in skin. The objectives: 1)To assess theproliferation of fibroblasts on the collagen gel by ESM peptides in vitro, 2) To evaluate fibroblasts migrationand enzyme activity (hyuaronase, tyrocinase, elastase etc) in vitro cell culture model. The main targets of thisproject are 1) enhance fibroblast proliferation, 2) anti-inflammation and 3) whitening. Fibroblast migration isimportant factor for maintaining skill elasticity, moisture and we measure the effect of ESM peptides on thecell proliferation and hyuaronase/estase activity. Anti-inflammatory activity will be measured by testing TNF-?,MCP-1, IL-6 which are typical inflammatory mediators in human skill and they are related to anti-aging ofskin. Lastly, we will measure whitening activity of ESM peptides by means of testing tyrocinase activity andanti-UV effect. The ultimate goal of the project is to develop valued added ESM peptides from industrial wasteESM. ESM derived biomaterials are attractive to world markets where individuals have religious concernsregarding porcine or bovine sourced material.