Value-added applications of eggshell membrane-derived bioactive peptides for cosmetic application

蛋壳膜生物活性肽在化妆品中的增值应用

• 批准号: 538393-2019
• 负责人: Mine, Yoshinori
• 金额: $ 0.91万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Engage Plus Grants Program
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=675335
• 关键词: Value; added; applications; eggshell; membrane

Canadian produces about 9 billion eggs per year and about 27% are processed at egg breaking which yield lowvalue eggshell as a by-product. The Canadian poultry industry produces about 1.5 tons eggshell membrane(ESM) waste annually. The discovery of novel value-added applications for eggshell waste would haveimportant financial and environmental benefits. Eggshell-derived collagen and collagen-derived peptides are anattractive replacement for bovine collagen due to the concern over bovine spongiform encephalopathy (BSE).Only a few applications have been reported, including matrices for adsorption of heavy metal ions, biologicaldressings for burns, substrates for cell culture and platforms for enzyme immobilization. Further studies arerequired to validate the biological activity of ESM peptides for specific cosmetic application. Hypothesis: ESMpeptides can be applied in cosmetics including skin grafts, stimulate the growth of human skin fibroblasts,moisture, whitening and reduce the production of inflammation in skin. The objectives: 1)To assess theproliferation of fibroblasts on the collagen gel by ESM peptides in vitro, 2) To evaluate fibroblasts migrationand enzyme activity (hyuaronase, tyrocinase, elastase etc) in vitro cell culture model. The main targets of thisproject are 1) enhance fibroblast proliferation, 2) anti-inflammation and 3) whitening. Fibroblast migration isimportant factor for maintaining skill elasticity, moisture and we measure the effect of ESM peptides on thecell proliferation and hyuaronase/estase activity. Anti-inflammatory activity will be measured by testing TNF-?,MCP-1, IL-6 which are typical inflammatory mediators in human skill and they are related to anti-aging ofskin. Lastly, we will measure whitening activity of ESM peptides by means of testing tyrocinase activity andanti-UV effect. The ultimate goal of the project is to develop valued added ESM peptides from industrial wasteESM. ESM derived biomaterials are attractive to world markets where individuals have religious concernsregarding porcine or bovine sourced material.

加拿大每年生产约90亿个鸡蛋，其中约27%是在鸡蛋破碎时加工的，这会产生低价值的蛋壳作为副产品。加拿大家禽业每年产生约1.5吨蛋壳膜(ESM)废物。蛋壳废料新的增值应用的发现将带来重要的经济和环境效益。由于对牛海绵状脑病(BSE)的关注，蛋壳胶原蛋白和胶原蛋白衍生多肽是一种有吸引力的牛胶原蛋白替代品，目前仅有少量应用报道，包括重金属离子吸附基质、烧伤生物敷料、细胞培养底物和酶固定化平台。还需要进一步的研究来验证ESM多肽在特定化妆品应用中的生物活性。假设：ESM多肽可以应用于包括皮肤移植在内的化妆品中，刺激人体皮肤成纤维细胞的生长，保湿，美白，并减少皮肤炎症的产生。目的：1)研究ESM多肽对成纤维细胞在胶原凝胶上的体外增殖作用；2)在体外细胞培养模型中，评价成纤维细胞的迁移和酶活性(透明质酸酶、酪氨酸酶、弹力酶等)。该项目的主要目标是1)促进成纤维细胞的增殖，2)抗炎和3)美白。成纤维细胞迁移是维持细胞弹性和水分的重要因素，我们测量了ESM多肽对细胞增殖和透明质酸酶/酯酶活性的影响。抗炎活性将通过检测肿瘤坏死因子-1、单核细胞趋化蛋白-1、白介素6来衡量，它们是人类技能中典型的炎症介质，它们与皮肤的抗衰老有关。最后，通过测定酪氨酸酶活性和抗紫外线作用来检测ESM多肽的美白活性。该项目的最终目标是从工业废渣ESM中开发高附加值的ESM多肽。ESM衍生的生物材料对世界市场很有吸引力，因为在世界市场上，个人对猪或牛来源的材料有宗教担忧。