Anti-inflammatory effects of a synthetic di-phosphoserine (PP2) egg yolk peptide in modulating inflammatory response

合成二磷酸丝氨酸 (PP2) 蛋黄肽在调节炎症反应中的抗炎作用

• 批准号: 453362-2013
• 负责人: Mine, Yoshinori
• 金额: $ 1.82万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=541449
• 关键词: Anti; inflammatory; effects; synthetic; di

Inflammation is now thought to be the central to etiology of many diseases. Phosphopeptides are one among the most interesting biomolecules and these functional bioactive peptides have shown the most promising anti-inflammatory potential as therapeutic or health promoting agents. Phosphopeptides usually contain clusters of phosphoserines, which have been shown earlier to exert immunomodulatory, anti-inflammatory and anti-oxidative stress effects. However, the role of phosphoserine residues- the key molecules that exert the function and their mechanism of action remain unknown. The role of a synthetic di-phosphoserine peptides, PP-2, in modulating inflammation will therefore be examined using inflammation induced in vitro models of Caco-2, HT-29 and RAW264.7 human cell lines. Potential signalling mechanisms via quantitative RT-PCR array and cytokine mediators of gene and protein expressions will be elucidated and characterized.

炎症现在被认为是许多疾病的核心病因。磷酸肽是最有趣的生物分子之一，这些功能性生物活性肽已显示出最有前途的抗炎治疗或健康促进剂的潜力。磷酸肽通常含有磷酸丝氨酸簇，其较早已显示出发挥免疫调节、抗炎和抗氧化应激作用。然而，磷酸丝氨酸残基的作用-发挥功能的关键分子及其作用机制仍然未知。因此，将使用Caco-2、HT-29和RAW264.7人类细胞系的炎症诱导体外模型来检查合成二磷酸丝氨酸肽PP-2在调节炎症中的作用。通过定量RT-PCR阵列和细胞因子介导的基因和蛋白质表达的潜在信号传导机制将被阐明和表征。