Cryopreservation of livestock colostrum-derived mesenchymal stem cells

家畜初乳来源的间充质干细胞的冷冻保存

• 批准号: 521869-2017
• 负责人: Elliott, Janet
• 金额: $ 1.82万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=640560
• 关键词: Cryopreservation; livestock; colostrum; derived; mesenchymal

Currently, antibiotics are widely used to prevent and control disease and infections in farm animals such as pigsand cows. However, the continued use of antibiotics in animals meant for human consumption can cause thegrowth of bacteria that become resistant to antibiotics. These harmful bacteria can also be passed on to humansvia the food chain. Health Canada is moving to phase out the use of some antibiotics in the animal industry tolimit the danger posed to the public by antibiotic-resistant bacteria in meat and other animal products.Therefore, new, safe and efficient strategies are needed to improve overall immunity and general health oflivestock by preventing disease/infection without the use of antibiotics. MySteM Biotechnologies Inc.(MySteMBiotech) is developing a new product composed of cells from the milk of pigs and cows that can beused instead of antibiotics. The source of the cells is the colostrum, the "first milk" released during the fewdays within the time the animals give birth. While it is widely known that colostrum is rich in antibodies, theirpreliminary results show that it also contains a special type of cells called mesenchymal stem cells (MSCs).These cells have the ability to boost the body's protective response against microorganisms that can causedisease. The company has successfully isolated these cells from pig and cow colostrum and grown them inculture to increase their numbers. They have also shown that these cells have the ability to wipe out bacteria inculture. Our expertise in the field of long-term storage of live cells and tissues will address the company's needfor strategies to freeze the cells so they can be kept alive for future use and in a form that can be readily andsafely given to farm animals. The enabling and innovative technology that is anticipated to result from thisstudy represents a natural and economic approach to enhance the health of Canada's livestock and boost ouragriculture and agri-food industry.

目前，抗生素被广泛用于预防和控制农场动物如猪和牛的疾病和感染。然而，在供人类食用的动物身上继续使用抗生素会导致细菌的生长，从而对抗生素产生抗药性。这些有害细菌也可以通过食物链传给人类。加拿大卫生部正在逐步淘汰畜牧业中某些抗生素的使用，以限制肉类和其他动物产品中的耐药性细菌对公众造成的危险。因此，需要新的、安全和有效的策略，通过在不使用抗生素的情况下预防疾病/感染来提高牲畜的整体免疫力和总体健康。MySteM生物技术公司目前，该公司正在开发一种新产品，它由来自猪和牛的奶中的细胞组成，可以用来代替抗生素。细胞的来源是初乳，即动物分娩后几天内释放的“第一口奶”。尽管众所周知初乳中富含抗体，但他们的初步研究结果显示，初乳中还含有一种特殊类型的细胞，称为间充质干细胞（MSCs）。这些细胞具有增强机体对致病微生物的保护反应的能力。该公司已经成功地从猪和牛的初乳中分离出这些细胞，并培养它们以增加它们的数量。他们还表明，这些细胞有能力消灭细菌培养。我们在活细胞和组织长期储存领域的专业知识将满足公司对冷冻细胞策略的需求，使它们能够保持活力以供将来使用，并以一种可以容易和安全地给予农场动物的形式。预计这项研究将产生的创新技术代表了一种自然和经济的方法，以提高加拿大牲畜的健康，促进农业和农业食品工业。