Droplet microfluidic system for production of high value coatings on luminescent nanoparticles

用于在发光纳米粒子上生产高价值涂层的液滴微流体系统

• 批准号: 479222-2015
• 负责人: Krull, Ulrich
• 金额: $ 12.22万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Strategic Projects - Group
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=641890
• 关键词: Droplet; microfluidic; system; production; value

There is tremendous demand for new diagnostic technologies by a wide variety of users as reflected by health care needs in hospitals and for home use, for the animal care sector associated with veterinary medicine, for food quality screening, and for environmental management. Many of the most competitive diagnostic technologies make use of nanomaterials, and in particular, conjugated nanoparticles. In collaboration with our industry partner GL Chemtec, our joint interest is to learn to efficiently manufacture modified nanoparticles and microbeads that will support the development of a variety of diagnostic applications. We propose to develop a two-phase water-in-oil droplet-based microfluidic platform that enables compartmentalization of individual reactions at kHz rates in a continuous flow manner, without the use of sophisticated control systems and the need to increase the device footprint. The basis of the proposed method is to encapsulate an individual polymer bead within a droplet so that immobilization and decoration of NPs on the bead will be done in a rigorously controlled reaction environment, followed by efficient recovery of the decorated nanoparticles. Beyond recovery of decorated nanoparticles from beads, such beads coated with nanoparticles will also be developed to provide the transduction component for competitive diagnostic platforms. The goal of this project is to develop a microfluidic platform with an order of magnitude higher manufacturing throughput than the best presently available, while concurrently providing flexibility in decoration of nanoparticles with a variety of conjugates by implementation of orthogonal chemistries. The proposed fluidics technology and reaction chemistries offer the capability of producing beads at a rate of thousands per second in continuous flow, offering scalable continuous production of practical quantities (milligrams to grams) of high quality decorated nanoparticles.

各种各样的用户对新的诊断技术有着巨大的需求，这反映在医院和家庭使用的医疗保健需求、与兽医学相关的动物护理部门、食品质量筛选和环境管理方面。许多最具竞争力的诊断技术利用纳米材料，特别是共轭纳米颗粒。通过与我们的行业合作伙伴GL Chemtec的合作，我们的共同兴趣是学习有效地制造改性纳米颗粒和微珠，以支持各种诊断应用的开发。我们建议开发一种基于两相油包水液滴的微流体平台，该平台能够以连续流动的方式以kHz速率对各个反应进行区室化，而无需使用复杂的控制系统和增加设备占地面积的需要。所提出的方法的基础是将单个聚合物珠包封在液滴内，使得珠上的NP的固定化和装饰将在严格控制的反应环境中进行，然后有效地回收装饰的纳米颗粒。除了从珠子中回收修饰的纳米颗粒外，还将开发涂有纳米颗粒的珠子，为竞争性诊断平台提供转导组件。该项目的目标是开发一种微流体平台，其制造吞吐量比目前最好的高一个数量级，同时通过正交化学的实施提供用各种缀合物装饰纳米颗粒的灵活性。所提出的射流技术和反应化学提供了以每秒数千的速率在连续流中生产珠粒的能力，提供了实际量（毫克至克）的高质量装饰纳米颗粒的可扩展连续生产。