Super-resolution radial fluctuation microscope for 4D imaging of cells and cellular dynamics

用于细胞和细胞动力学 4D 成像的超分辨率径向波动显微镜

• 批准号: RTI-2018-00080
• 负责人: Dellaire, Graham
• 金额: $ 6.27万
• 依托单位: Dalhousie University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=642158
• 关键词: Super; resolution; radial; fluctuation; microscope

Our 6 research groups use a state-of-the-art custom-built spinning-disk confocal microscopy originally funded by investments from both CFI and NSERC. This microscope is cable of both fixed and high-speed live-cell imaging that is central to our research programs, and is used to observe a wide-array of proteins (e.g. mitochondrial, DNA repair and RNA-binding factors), cellular processes (e.g. DNA repair, synapse formation, autophagy, host-pathogen interactions) and structures (e.g. nuclear subdomains, synapses, mitochondria, autophagosomes and stress granules) within several different organisms including protists, bacteria, viruses, and both human and zebrafish cells.

我们的6个研究小组使用最先进的定制旋转盘共聚焦显微镜，最初由CFI和NSERC投资资助。这种显微镜是电缆固定和高速活细胞成像是我们的研究计划的核心，并用于观察广泛的蛋白质阵列（例如线粒体、DNA修复和RNA结合因子）、细胞过程（例如DNA修复、突触形成、自噬、宿主-病原体相互作用）和结构在包括原生生物、细菌、病毒以及人类和斑马鱼细胞在内的几种不同生物体中，细胞核的结构域（例如，核亚结构域、突触、线粒体、自噬体和应激颗粒）可以是细胞核的一部分。