Scanning histology and immunohistochemistry-prepared tissue for bridging across scales of molecular, cellular, systems and imaging neuroscience

扫描组织学和免疫组织化学制备的组织，以跨越分子、细胞、系统和成像神经科学的尺度

• 批准号: RTI-2018-00953
• 负责人: Shmuel, Amir
• 金额: $ 10.93万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=642172
• 关键词: Scanning; histology; immunohistochemistry; prepared; tissue

We request a cutting edge system for high throughput, high resolution optical scanning of brain tissue. Prior to the scanning, the tissue is extracted from brains of experimental animals, sectioned into slices that are mounted on slides, and processed according to various histology, immunofluorescence or immunohistochemistry protocols. We will also use the scanner to validate the selective expression of optogenetic constructs and to validate fluorescence expression in transgenic/Cre animal lines.

我们需要一个尖端的系统，用于高通量、高分辨率的脑组织光学扫描。在扫描之前，从实验动物的大脑中提取组织，切成切片，装在载玻片上，并根据各种组织学，免疫荧光或免疫组织化学协议进行处理。我们还将使用扫描仪验证光遗传构建体的选择性表达，并验证转基因/Cre动物系中的荧光表达。