On-Column Fluorescence Detection for High-Throughput Identification of Cysteine Reactive Chemicals in the Environment

近几十年来，加拿大和世界范围内癌症和糖尿病等疾病发病率的增加与环境污染有关。然而，由于大多数环境化学品的毒性仍未查明，因此对环境化学品的健康风险进行预防性评估仍有许多不足之处。考虑到目前以动物为基础的毒性试验的时间和财务成本，对大量环境化学品（超过70,000种用于商业用途）的毒性进行系统调查是不可能的。氧化应激是一种特别关注的常见毒性，因为它被认为是饮用水中空气颗粒物（PM）和消毒副产物（DBPs）的主要毒性。迫切需要开发高通量方法来识别PM和饮用水中引起氧化应激的有毒化学物质。

Increasing incidence of diseases, such as cancer and diabetes, in Canada and worldwide in recent decades has been associated with environmental pollution. However, preventative assessment of the health risks of environmental chemicals leaves much to be desired since the toxicities for most environmental chemicals remain unidentified. Systematic investigation of the toxicities of immense number of environmental chemicals (over 70,000 in commercial use) is implausible given the time and financial cost of current animal-based toxicity tests. Oxidative stress is a common toxicity of special concern since it is proposed as the primary toxicity of air particulate matter (PM) and disinfection by-products (DBPs) in drinking water. Development of high-throughput methods to identify the toxic chemicals that cause oxidative stress in PM and drinking water is in urgent need.