On-Column Fluorescence Detection for High-Throughput Identification of Cysteine Reactive Chemicals in the Environment

用于高通量鉴定环境中半胱氨酸反应性化学物质的柱上荧光检测

• 批准号: RTI-2018-00871
• 负责人: Peng, Hui
• 金额: $ 10.93万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=642234
• 关键词: Column; Fluorescence; Detection; Throughput; Identification

Increasing incidence of diseases, such as cancer and diabetes, in Canada and worldwide in recent decades has been associated with environmental pollution. However, preventative assessment of the health risks of environmental chemicals leaves much to be desired since the toxicities for most environmental chemicals remain unidentified. Systematic investigation of the toxicities of immense number of environmental chemicals (over 70,000 in commercial use) is implausible given the time and financial cost of current animal-based toxicity tests. Oxidative stress is a common toxicity of special concern since it is proposed as the primary toxicity of air particulate matter (PM) and disinfection by-products (DBPs) in drinking water. Development of high-throughput methods to identify the toxic chemicals that cause oxidative stress in PM and drinking water is in urgent need.

近几十年来，癌症和糖尿病等疾病在加拿大和世界各地的发病率不断上升，这与环境污染有关。然而，对环境化学品的健康风险进行预防性评估仍有许多不足之处，因为大多数环境化学品的毒性仍未确定。考虑到目前动物毒性测试的时间和经济成本，对大量环境化学品(商业用途中超过70000种)的毒性进行系统调查是不可能的。氧化应激被认为是饮用水中空气颗粒物(PM)和消毒副产物(DBPs)的主要毒性，因此是一种特别值得关注的常见毒性。迫切需要开发高通量方法来识别导致PM和饮用水中氧化应激的有毒化学物质。