On-Column Fluorescence Detection for High-Throughput Identification of Cysteine Reactive Chemicals in the Environment

近几十年来，加拿大和全世界癌症和糖尿病等疾病的发病率不断增加，这与环境污染有关。然而，对环境化学品的健康风险进行预防性评估仍有许多不足之处，因为大多数环境化学品的毒性仍未确定。鉴于目前基于动物的毒性试验的时间和经济成本，对大量环境化学品（商业用途超过70，000种）的毒性进行系统调查是不可能的。氧化应激是一种特别关注的常见毒性，因为它被认为是饮用水中空气颗粒物（PM）和消毒副产物（DBPs）的主要毒性。迫切需要开发高通量方法来鉴定导致PM和饮用水中氧化应激的有毒化学物质。

Increasing incidence of diseases, such as cancer and diabetes, in Canada and worldwide in recent decades has been associated with environmental pollution. However, preventative assessment of the health risks of environmental chemicals leaves much to be desired since the toxicities for most environmental chemicals remain unidentified. Systematic investigation of the toxicities of immense number of environmental chemicals (over 70,000 in commercial use) is implausible given the time and financial cost of current animal-based toxicity tests. Oxidative stress is a common toxicity of special concern since it is proposed as the primary toxicity of air particulate matter (PM) and disinfection by-products (DBPs) in drinking water. Development of high-throughput methods to identify the toxic chemicals that cause oxidative stress in PM and drinking water is in urgent need.