Replacement for Microbial Growth Assay Cluster

微生物生长检测簇的替代品

• 批准号: RTI-2019-00596
• 负责人: AbouElela, Sherif
• 金额: $ 10.44万
• 依托单位: Université de Sherbrooke
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=643363
• 关键词: Replacement; Microbial; Growth; Assay; Cluster

The capacity to analyse cell growth is the cornerstone of most genetic screens and an essential step for characterizing gene function and contribution to cell biology. Growth patterns in liquid media of single cell organisms like bacteria and yeast reveals essential information about how different genes contribute to cell function. Indeed, growth curves reveal key insights about the physiological and metabolic functions of singles cell organisms. Using a unique cluster of microplate spectrophotometers equipped with shaker and real time sampling fitted with our home-made growth analysis software we performed thousands of growth curves of yeast and bacterial cells for tens of researchers at the Université de Sherbrooke, and coached dozens of graduate students in their studies of gene and cell function. The goal of this application is to replace our defective liquid growth curve analysis cluster in order to maintain our phenotypic platform as a service to all researchers at the Université de Sherbrooke. It is essential for our institution to keep its capacity to provide microbial cells analysis solution in order to maintain the NSERC-funded research programs using bacterial and yeast model systems. ******We are requesting funds to replace a cluster of 12 BioTek PowerWave plate-readers that we have used for the last 16 years to support the study of yeast and bacterial cell growth with a cluster of 8 modern high-capacity machines. Our current plate-readers are discontinued, and spare parts are no longer available for repairs. There are no systems with similar configurations available on campus and without them, the whole pipeline of growth assays and analyses essential to complete our NSERC-funded research will cease to function. The new machines will ensure continued service to the 10 labs at the faculty of medicine and tens of different research group across the Université de Sherbrooke. Most importantly, the requested equipment will permit the continuation of our unique graduate training programs, which was recently funded by NSERC CREATE program. In summary, the requested machines are absolutely needed as replacements for basic equipment essential for supporting the research and training programs funded by NSERC.*****

分析细胞生长的能力是大多数遗传筛选的基石，也是确定基因功能和对细胞生物学的贡献的关键步骤。细菌和酵母等单细胞生物体在液体介质中的生长模式揭示了关于不同基因如何影响细胞功能的基本信息。事实上，生长曲线揭示了关于单细胞生物体的生理和代谢功能的关键见解。我们使用独特的微型平板光谱仪集群，配备振动器和与我们自制的生长分析软件相匹配的实时采样，为舍布鲁克大学的数十名研究人员绘制了数千条酵母和细菌细胞的生长曲线，并指导了数十名研究生进行基因和细胞功能的研究。该应用程序的目标是替换我们有缺陷的液体生长曲线分析集群，以维护我们的表型平台，为舍布鲁克大学的所有研究人员提供服务。对于我们的机构来说，保持其提供微生物细胞分析解决方案的能力是至关重要的，以维持NSERC资助的使用细菌和酵母模型系统的研究计划。*我们正在申请资金，将过去16年来我们一直用于支持酵母和细菌细胞生长研究的12台BioTek PowerWave平板阅读器更换为8台现代高容量机器的集群。我们目前的读板机已停产，备件也不能再维修了。校园里没有类似配置的系统，如果没有它们，完成我们NSERC资助的研究所必需的整个增长分析和分析管道将停止运行。新机器将确保继续为舍布鲁克大学医学院的10个实验室和数十个不同的研究小组提供服务。最重要的是，所要求的设备将使我们独特的研究生培训计划得以继续，该计划最近由NSERC Create计划资助。总之，所要求的机器绝对需要作为支持NSERC资助的研究和培训计划所必需的基本设备的替代品。*