Discovery and application of regulatory mechanisms controlling SNF1 kinase dependent stress resistance

控制SNF1激酶依赖性应激抵抗的调控机制的发现和应用

• 批准号: RGPIN-2016-04575
• 负责人: Arnason, Terra
• 金额: $ 2.26万
• 依托单位: University of Saskatchewan
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=644145
• 关键词: Discovery; application; regulatory; mechanisms; controlling

Global weather extremes and population pressures on world food supply, combined with decreasing arable land availability and declining ocean stocks, underscore the urgency in understanding how to enhance food production in suboptimal environments. Critical to stress resistance is the SNF1 kinase enzyme complex. Our earlier work with yeast cells showed that SNF1 kinase activity is important in promoting the cells' responses to environmental stress and lack of nutrients, helping them to adapt to poor conditions. Conserved versions of SNF1 enzymes are found in all plants and animals. Understanding how their action is regulated genetically, and how it helps organisms to survive, offers the potential to develop crops and livestock that are better adapted to thrive in harsh environments. Our short term goals aim to provide evidence that food production can be enhanced through SNF1 kinase activity, and to identify targets for future approaches to the same end. ******Our short term goals will be addressed by the following three objectives:***Objective 1: To establish the significance of enhanced Snf1 activity on stress resistance in plants***Objective 2: To establish the significance of enhanced Snf1 activity on stress resistance in fish***Objective 3: To perform a large-scale yeast screen to comprehensively identify activators and repressors of SNF1 kinase including those involved in nuclear-cytosolic segregation. ******Part of our work involves an intensive search that will identify new methods of activating this enzyme. Another aspect of the proposed work is to directly test how a known overactive enzyme will provide survival benefits when exposed to realistic stresses. Within this funding cycle, we will measure how the genetic enhancement of SNF1 kinase activity augments plant and fish survival when they are exposed to common stressors associated with poor production environments. Arabidopsis, a brassica related to canola, cabbage and other food crops, and zebrafish will be used to test survivability and health when SNF1 kinase activity is augmented. Plants will be exposed to conditions of low soil nutrients, high salinity and heavy metal contamination. Fish will be subjected to temperature variations, low nutrients, variable levels of ammonia waste, and human handling. In the long term, such benefits will be translated to the engineering of more robust agricultural and aquacultural strains that will have positive impacts on world food supply. This will also specifically benefit Canada through the creation of stress resistant Atlantic salmon or rainbow trout, or stress resistant canola and legumes, which are valuable economic industries.**

全球极端天气和人口对世界粮食供应的压力，加上可耕地减少和海洋资源减少，突出表明迫切需要了解如何在次优环境中提高粮食生产。SNF 1激酶复合物是抗逆的关键。我们早期对酵母细胞的研究表明，SNF 1激酶活性在促进细胞对环境压力和营养缺乏的反应方面非常重要，有助于它们适应恶劣的条件。SNF 1酶的保守版本存在于所有植物和动物中。了解它们的行为如何在基因上受到调节，以及它如何帮助生物体生存，可以开发出更适合在恶劣环境中茁壮成长的作物和牲畜。我们的短期目标旨在提供证据证明可以通过SNF 1激酶活性提高粮食产量，并确定未来实现同一目标的方法的目标。** 我们的短期目标将通过以下三个目标来实现：* 目标1：确定增强的Snf 1活性对植物抗逆性的意义 * 目标2：确定增强的Snf 1活性对鱼类抗逆性的意义 * 目标3：进行大规模酵母筛选，以全面鉴定SNF 1激酶的激活子和阻遏子，包括参与核-胞质分离的那些。** 我们的部分工作涉及深入研究，以确定激活这种酶的新方法。拟议工作的另一个方面是直接测试一种已知的过度活性酶在暴露于现实压力时如何提供生存益处。在此资助周期内，我们将测量SNF 1激酶活性的遗传增强如何增强植物和鱼类在暴露于与恶劣生产环境相关的常见压力源时的存活率。 拟南芥，一种与油菜、卷心菜和其他粮食作物有关的芸苔属植物，以及斑马鱼将被用来测试SNF 1激酶活性增强时的生存能力和健康状况。植物将暴露在土壤养分低、盐度高和重金属污染的条件下。鱼将受到温度变化，低营养，可变水平的氨废物，和人类处理。 从长远来看，这些好处将转化为更强大的农业和水产养殖品种的工程，这将对世界粮食供应产生积极影响。这也将特别有利于加拿大通过创造抗应激大西洋鲑鱼或虹鳟鱼，或抗应激油菜和豆类，这是有价值的经济产业。