Analysis of the role of the ubiquitin system in the regulation of yeast carbohydrate metabolism: the necessity of a bench top centrifuge

泛素系统在酵母碳水化合物代谢调节中的作用分析：台式离心机的必要性

• 批准号: 359126-2008
• 负责人: Arnason, Terra
• 金额: $ 1.99万
• 依托单位: University of Saskatchewan
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2007
• 资助国家: 加拿大
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=362064
• 关键词: Analysis; role; ubiquitin; system; regulation

Every organism, whether a human or a single cell, has the ability to adapt its metabolism between times of feast or famine. Abundant nutrients enable energy stores to be laid down and later released when times are lean. Cells and organisms efficiently respond to nutrient abundance by altering the operation of their metabolic pathways through the rapid and reversible activation of key enzymes that are turned on or off in response to nutrient signals. Our studies focus on the evolutionarily conserved yeast enzyme, SNF1 kinase, which turns energy consuming metabolic pathways off to preserve a constant energy level in times of need. This simple yeast system will be used to explore the molecular regulation of how a cell responds to limiting nutrients. To study nutrient response and metabolism in yeast, it is necessary to grow large batches of cells in specialized liquid growth media. The cells must then be purified from the media using a centrifuge. A centrifuge is vital to the work as it enables microorganisms to be purified and concentrated from large cultures for analysis. Without this equipment, research laboratories, especially the trainees, will not be able to move forward in their studies. With the availability of a centrifuge in my laboratory, we expect to be able to grow enough yeast cells to study the specific regulatory mechanisms that enable SNF1 to protect the cell when nutrients are limiting. Understanding how SNF1 kinase activity is controlled in yeast may lead to understanding regulation of human metabolism. Interestingly, the human SNF1 kinase, the AMPK kinase, is suboptimal in metabolic syndrome, which encompasses type 2 diabetes and obesity. Knowing how the AMPK family can be activated may lead to ways of correcting the metabolic dysfunction in metabolic syndrome.

每一种生物，无论是人类还是单个细胞，都有能力在丰年和饥荒之间调整自己的新陈代谢。丰富的营养物质使能量储备得以储存，并在时间贫乏时释放出来。细胞和生物体通过快速和可逆的关键酶的激活来改变其代谢途径的操作，从而有效地响应营养丰富，这些酶在响应营养信号时被打开或关闭。我们的研究集中在进化上保守的酵母酶，SNF1激酶，它关闭能量消耗代谢途径，在需要的时候保持恒定的能量水平。这个简单的酵母系统将被用来探索细胞如何对限制营养作出反应的分子调节。为了研究酵母的营养反应和代谢，有必要在专门的液体培养基中培养大量细胞。然后必须用离心机从培养基中纯化细胞。离心机对这项工作至关重要，因为它可以从大型培养物中纯化和浓缩微生物以供分析。没有这些设备，研究实验室，特别是受训者，将无法在研究中取得进展。随着实验室离心机的可用性，我们期望能够培养足够的酵母细胞来研究特定的调节机制，使SNF1在营养受限时保护细胞。了解酵母中SNF1激酶活性是如何被控制的，将有助于理解人类代谢的调控。有趣的是，人类SNF1激酶，AMPK激酶，在代谢综合征（包括2型糖尿病和肥胖）中是次优的。了解AMPK家族如何被激活可能会导致纠正代谢综合征代谢功能障碍的方法。