Nanoparticle Tracking Analysis (NTA) System for Quantification and Analysis of Biologically Relevant Nanosized Particles

用于生物相关纳米颗粒定量和分析的纳米颗粒跟踪分析 (NTA) 系统

• 批准号: RTI-2019-00534
• 负责人: Christian, Sherri
• 金额: $ 10.93万
• 依托单位: Memorial University of Newfoundland
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=647663
• 关键词: Nanoparticle; Tracking; Analysis; NTA; System

Analysis and use of nanoparticles is an emerging and important area of research. Nanoparticles have diameters in the nanometer range, which is less than 1/1000th of a millimeter. These particles are so small that they cannot be seen using typical instruments or microscopes because of the limits of detection imposed by the wavelength of light. In biological sciences research, nanoparticles include viruses and viral-like particles, lipid-protein complexes like LDL or HDL, neuronal synaptosomes, as well a newly discovered nanoparticles called extracellular vesicles (EVs). EVs are a new way that many cells, including cancer cells, immune cells, and bacteria, use to communicate with each other. It is critical that researchers at Memorial University who study the importance of biological nanoparticles can accurately and precisely quantify and characterize these unique biological objects.******Nanoparticle tracking analysis (NTA) uses the light-scattering of nanoparticles by their Brownian motion to overcome the limits of detection imposed by the wavelengths of light to visualize, size, and quantify biological nanoparticles. Recent advances in fluorescence detection technology also allows researchers to combine NTA with specific detection of proteins or lipids to define sub-populations of EVs, viruses, or lipoproteins. The only technique that is able to accurately and rapidly visualize, size, and quantify mixed populations of nanoparticles is NTA, and the only NTA instrument with fluorescence detection capabilities is the NanoSight NS300 from Malvern Instruments.******The NanoSight NS300 will directly support the research programs of the 10 applicants from the Departments of Biochemistry, Biology, and Ocean Sciences in the Faculty of Science as well as from the Division of BioMedical Sciences in the Faculty of Medicine. In addition, at least four other research groups have a significant need for this NTA technology. The work performed by these groups spans life sciences research from virology to immunology, from neurobiology to aquaculture to nutrition, and more. The applicants and other users of the NS300 will analyze and quantify EVs including exosomes, microvesicles and synaptosomes, as well as lipid droplets, virus particles, experimental lipoprotein preparations, and mitochondria. Thus, acquisition of the NS300 will directly benefit multiple and diverse research programs across Memorial University allowing them to compete on a global stage to provide new and exciting answers to some of life's most fundamental questions.**

纳米粒子的分析和使用是一个新兴的重要研究领域。纳米颗粒的直径在纳米范围内，小于1/1000毫米。这些粒子是如此之小，以至于它们无法使用典型的仪器或显微镜看到，因为光的波长所施加的检测极限。在生物科学研究中，纳米颗粒包括病毒和病毒样颗粒，脂质-蛋白质复合物如LDL或HDL，神经元突触体，以及新发现的称为细胞外囊泡（EV）的纳米颗粒。电动汽车是许多细胞（包括癌细胞、免疫细胞和细菌）用来相互交流的一种新方式。至关重要的是，纪念大学研究生物纳米粒子重要性的研究人员能够准确，精确地量化和表征这些独特的生物物体。纳米粒子跟踪分析（NTA）利用纳米粒子的布朗运动进行光散射，克服光波长对检测的限制，从而对生物纳米粒子进行可视化、尺寸确定和定量。荧光检测技术的最新进展也使研究人员能够将联合收割机NTA与蛋白质或脂质的特异性检测相结合，以定义EV、病毒或脂蛋白的亚群。唯一能够准确和快速地可视化、测量和定量纳米颗粒混合群体的技术是NTA，唯一具有荧光检测能力的NTA仪器是来自马尔文仪器公司的NanoSight NS 300。NanoSight NS 300将直接支持来自理学院生物化学，生物学和海洋科学系以及医学院生物医学科学系的10名申请人的研究计划。此外，至少有四个其他研究小组对这种NTA技术有很大的需求。这些小组所做的工作涵盖了从病毒学到免疫学、从神经生物学到水产养殖再到营养学等生命科学研究。NS 300的申请人和其他用户将分析和定量EV，包括外泌体、微泡和突触体，以及脂滴、病毒颗粒、实验脂蛋白制剂和线粒体。因此，收购NS 300将直接有利于纪念大学的多个不同的研究项目，使他们能够在全球舞台上竞争，为生活中一些最基本的问题提供新的和令人兴奋的答案。