Genomic, transcriptomic and culture-based assessment of UV irradiation effects on phytoplankton viability: applications for ballast water treatment systems

美国海岸警卫队（USCG）最近出台了严格的压载水处理条例，以尽量减少或消除通过压载水运输引入水生入侵物种的风险。紫外线（UV）照射是压载水处理系统（BWTS）** 中的一项有用技术，用于消除可能对人类和环境有害的水生物种。紫外线 ** 会破坏DNA并阻止细胞复制，但USCG协议 ** 中规定的重要染色方法可能无法检测到浮游植物中的紫外线损伤。由于对基于文化的物种偏见（即，并非所有物种都能生长）。快速和准确的检测，以确定细胞的紫外线损坏无法恢复是至关重要的接受紫外线为基础的BWTS的浮游植物。因此，我们建议开发高通量浮游植物培养 ** 技术，并将其与基因组/转录组学方法相结合，以量化DNA损伤和 ** 相关的基因转录中断，这些基因转录发生在 ** 通常在压载水中发现的浮游植物群落中的一系列基因功能类别的关键功能基因位点。特洛伊科技公司（Trojan），总部位于加拿大安大略的伦敦，是全球领先的水处理公司之一。特洛伊公司提供的产品和服务在使水处理过程的各个阶段更加有效，高效和可持续方面发挥着至关重要的作用，从住宅到工业，市政和海洋应用。紫外线杀菌技术在BWTS ** 上的应用代表了一个超过150亿美元的潜在全球市场。我们将与Trojan合作开发 ** 高通量培养方案，结合先进的元基因组和元转录组技术 **，以验证BWTS的紫外线灭菌，从而在全球范围内扩大Trojan的市场，并为 ** 航运业提供实用，安全和具有成本效益的压载水灭菌选择。

The United States Coast Guard (USCG) recently introduced stringent regulations for the treatment of ballast**water to minimize or eliminate the risk of the introduction of aquatic invasive species via ballast water**transport. Ultra-violet (UV) light irradiation is a useful technology in ballast water treatment systems (BWTSs)**for eliminating aquatic species which could be invasive and harmful to humans and the environment. UV**damages DNA and prevents cellular replication, but the vital stain methods mandated in the USCG protocol**may not detect UV damage in phytoplankton. Alternative culture-based measures of reproductive capacity are**yet to be approved due to concerns over culture-based species biases (i.e., not all species may grow). Rapid and**accurate assays to identify cells that are UV damaged beyond recovery are critical for the acceptance of**UV-based BWTSs for phytoplankton. Hence we propose to develop high-throughput phytoplankton culture**technologies and combine them with genomic/transcriptomic approaches to quantify DNA damage and the**associated disruption of gene transcription at key functional gene loci across a range of gene function classes in**the phytoplankton community typically found in ballast water.**Trojan Technologies (Trojan), headquartered in London, Ontario, Canada is one of the world's leading water**treatment companies. The products and services provided by Trojan companies play vital roles in making**various stages of the water treatment process more effective, efficient and sustainable, ranging from residential**to industrial, municipal and marine applications. The application of UV sterilization technology to BWTSs**represents a potential global market of >$15 billion (USD). We will partner with Trojan to develop the**high-throughput culture protocols in concert with advanced meta-genomic and meta-transcriptomic technology**to validate UV sterilization for BWTSs and thus expand Trojan's market on a global scale and provide the**shipping industry with a practical, safe and cost-effective ballast water sterilization option.