Genomic, transcriptomic and culture-based assessment of UV irradiation effects on phytoplankton viability: applications for ballast water treatment systems
紫外线照射对浮游植物活力影响的基因组、转录组和基于培养的评估:压载水处理系统的应用
基本信息
- 批准号:516534-2017
- 负责人:
- 金额:$ 1.03万
- 依托单位:
- 依托单位国家:加拿大
- 项目类别:Collaborative Research and Development Grants
- 财政年份:2019
- 资助国家:加拿大
- 起止时间:2019-01-01 至 2020-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The United States Coast Guard (USCG) recently introduced stringent regulations for the treatment of ballastwater to minimize or eliminate the risk of the introduction of aquatic invasive species via ballast watertransport. Ultra-violet (UV) light irradiation is a useful technology in ballast water treatment systems (BWTSs)for eliminating aquatic species which could be invasive and harmful to humans and the environment. UVdamages DNA and prevents cellular replication, but the vital stain methods mandated in the USCG protocolmay not detect UV damage in phytoplankton. Alternative culture-based measures of reproductive capacity areyet to be approved due to concerns over culture-based species biases (i.e., not all species may grow). Rapid andaccurate assays to identify cells that are UV damaged beyond recovery are critical for the acceptance ofUV-based BWTSs for phytoplankton. Hence we propose to develop high-throughput phytoplankton culturetechnologies and combine them with genomic/transcriptomic approaches to quantify DNA damage and theassociated disruption of gene transcription at key functional gene loci across a range of gene function classes inthe phytoplankton community typically found in ballast water.Trojan Technologies (Trojan), headquartered in London, Ontario, Canada is one of the world's leading watertreatment companies. The products and services provided by Trojan companies play vital roles in makingvarious stages of the water treatment process more effective, efficient and sustainable, ranging from residentialto industrial, municipal and marine applications. The application of UV sterilization technology to BWTSsrepresents a potential global market of >$15 billion (USD). We will partner with Trojan to develop thehigh-throughput culture protocols in concert with advanced meta-genomic and meta-transcriptomic technologyto validate UV sterilization for BWTSs and thus expand Trojan's market on a global scale and provide theshipping industry with a practical, safe and cost-effective ballast water sterilization option.
美国海岸警卫队(USCG)最近推出了严格的压载水处理法规,以尽量减少或消除通过压载水运输引入水生入侵物种的风险。紫外线(UV)光照射是压载水处理系统(BWTS)中用于消除可能对人类和环境具有侵入性和有害的水生物种的有用技术。紫外线损伤DNA并阻止细胞复制,但USCG协议中规定的重要染色方法可能无法检测到浮游植物中的紫外线损伤。由于对基于文化的物种偏见(即,并非所有物种都能生长)。快速和准确的检测,以确定细胞的紫外线损伤超过恢复是至关重要的接受紫外线为基础的BWTS的浮游植物。因此,我们建议开发高通量的浮游植物培养技术和联合收割机,他们与基因组/转录组学的方法来量化DNA损伤和相关的基因转录的破坏在关键功能基因位点的浮游植物群落中的一系列基因功能类通常发现在压载水Trojan Technologies(特洛伊),总部设在安大略伦敦,加拿大是世界领先的水处理公司之一。特洛伊公司提供的产品和服务在使水处理过程的各个阶段更加有效,高效和可持续方面发挥着至关重要的作用,从住宅到工业,市政和海洋应用。紫外线杀菌技术在BWTS上的应用代表了一个超过150亿美元的潜在全球市场。我们将与Trojan合作开发高通量培养方案,结合先进的元基因组和元转录组技术,以验证BWTS的紫外线灭菌,从而在全球范围内扩大Trojan的市场,并为航运业提供实用,安全和具有成本效益的压载水灭菌选择。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Heath, Daniel其他文献
Environmental factors associated with reproductive barrier breakdown in sympatric trout populations on Vancouver Island
- DOI:
10.1111/j.1752-4571.2009.00100.x - 发表时间:
2010-01-01 - 期刊:
- 影响因子:4.1
- 作者:
Heath, Daniel;Bettles, Cory M.;Roff, Derek - 通讯作者:
Roff, Derek
Domestic-wild hybridization to improve aquaculture performance in Chinook salmon
- DOI:
10.1016/j.aquaculture.2019.734255 - 发表时间:
2019-09-15 - 期刊:
- 影响因子:4.5
- 作者:
Semeniuk, Christina A. D.;Capelle, Pauline M.;Heath, Daniel - 通讯作者:
Heath, Daniel
Assessing high-throughput environmental DNA extraction methods for meta-barcode characterization of aquatic microbial communities
- DOI:
10.2166/wh.2018.108 - 发表时间:
2019-02-01 - 期刊:
- 影响因子:2.3
- 作者:
Shahraki, Abdolrazagh Hashemi;Chaganti, Subba Rao;Heath, Daniel - 通讯作者:
Heath, Daniel
Heath, Daniel的其他文献
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{{ truncateString('Heath, Daniel', 18)}}的其他基金
Adaptive transgenerational signaling; maternal and paternal effects on early life history in salmon
自适应跨代信号传导;
- 批准号:
RGPIN-2019-06792 - 财政年份:2022
- 资助金额:
$ 1.03万 - 项目类别:
Discovery Grants Program - Individual
Adaptive transgenerational signaling; maternal and paternal effects on early life history in salmon
自适应跨代信号传导;
- 批准号:
RGPIN-2019-06792 - 财政年份:2021
- 资助金额:
$ 1.03万 - 项目类别:
Discovery Grants Program - Individual
Adaptive transgenerational signaling; maternal and paternal effects on early life history in salmon
自适应跨代信号传导;
- 批准号:
RGPIN-2019-06792 - 财政年份:2020
- 资助金额:
$ 1.03万 - 项目类别:
Discovery Grants Program - Individual
Adaptive transgenerational signaling; maternal and paternal effects on early life history in salmon
自适应跨代信号传导;
- 批准号:
RGPIN-2019-06792 - 财政年份:2019
- 资助金额:
$ 1.03万 - 项目类别:
Discovery Grants Program - Individual
Evolutionary Genetics of Early Life History Variation in Salmon: Non-additive Genetic Effects and Local Adaptation
鲑鱼早期生活史变异的进化遗传学:非加性遗传效应和局部适应
- 批准号:
RGPIN-2014-06469 - 财政年份:2018
- 资助金额:
$ 1.03万 - 项目类别:
Discovery Grants Program - Individual
Genomic, transcriptomic and culture-based assessment of UV irradiation effects on phytoplankton viability: applications for ballast water treatment systems
紫外线照射对浮游植物活力影响的基因组、转录组和基于培养的评估:压载水处理系统的应用
- 批准号:
516534-2017 - 财政年份:2018
- 资助金额:
$ 1.03万 - 项目类别:
Collaborative Research and Development Grants
Great Lakes Recreational Water Security: Microbial community characterization, source tracking and remediation through meta-genomics
五大湖休闲用水安全:通过元基因组学进行微生物群落表征、源头追踪和修复
- 批准号:
479134-2015 - 财政年份:2017
- 资助金额:
$ 1.03万 - 项目类别:
Strategic Projects - Group
Evolutionary Genetics of Early Life History Variation in Salmon: Non-additive Genetic Effects and Local Adaptation
鲑鱼早期生活史变异的进化遗传学:非加性遗传效应和局部适应
- 批准号:
RGPIN-2014-06469 - 财政年份:2017
- 资助金额:
$ 1.03万 - 项目类别:
Discovery Grants Program - Individual
REGENERATE: Restorative Genomic Engineering to Remediate Aquatic Threats to Ecosystems - Development
REGENERATE:恢复性基因组工程以修复对生态系统的水生威胁 - 开发
- 批准号:
514024-2017 - 财政年份:2017
- 资助金额:
$ 1.03万 - 项目类别:
Discovery Frontiers - Biodiversity and Adaptation of Biosystems
Genomic, transcriptomic and culture-based assessment of UV irradiation effects on phytoplankton viability: applications for ballast water treatment systems
紫外线照射对浮游植物活力影响的基因组、转录组和基于培养的评估:压载水处理系统的应用
- 批准号:
516534-2017 - 财政年份:2017
- 资助金额:
$ 1.03万 - 项目类别:
Collaborative Research and Development Grants
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