Biomarkers and bioremediation solutions for 1,4-dioxane contamination

1,4-二恶烷污染的生物标记物和生物修复解决方案

• 批准号: 530092-2018
• 负责人: Hug, Laura
• 金额: $ 3.07万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=654058
• 关键词: Biomarkers; bioremediation; solutions; dioxane; contamination

1,4-dioxane is a common groundwater contaminant and likely human carcinogen. As such, 1,4-dioxane**represents an environmental and human health hazard. Dioxane is a common co-contaminant in many active**and retired industrial sites where it can prevent remediation of co-occurring, high-priority contaminants such as**chlorinated solvents. Understanding the capacity for 1,4-dioxane attenuation and bioremediation at a**contaminated site is thus important to environmental protection agencies, site owners, and the general public.**The proposed research has concurrent aims of identifying biomarkers for microbially-mediated 1,4-dioxane**degradation and development of 1,4-dioxane degrading enrichment cultures as potential bioremediation tools.**The work will include field sampling two 1,4-dioxane contaminated sites - a southern Ontario landfill and a**former chemical plant in Southeast Texas. Microbial biomass will be filtered from landfill leachate and**groundwater. Environmental microbes will be characterized through metagenomic sequencing, and 1,4-dioxane**degraders cultivated and their activities characterized. Genes involved in 1,4-dioxane degradation will be**identified from the sampled sites as well as from public metagenome sequences from diverse environments,**defining the prevalence and potential for 1,4-dioxane degradation across the globe. Accurate site assessment**and access to cost-effective bioremediation options will have strong economic benefits for site owners. Based**on dioxane's toxicity and prevalence, maximum contaminant levels are expected to be implemented by**Environment Canada and the U.S. Environmental Protection Agency within the next few years. Development**of biomarkers to assess the potential for 1,4-dioxane degradation and establishment of bioremediation tools are**thus proactive steps to ensure Canadian policy makers and site owners have rapid, appropriate responses to**newly established maximum contaminant levels.

1，4-二氧六环是一种常见的地下水污染物，可能是人类致癌物。因此，1，4-二氧六环 ** 对环境和人类健康具有危害。二氧杂环己烷是许多活跃 ** 和废弃工业场地中常见的共同污染物，在这些场地中，二氧杂环己烷会妨碍对氯化溶剂等共同存在的高优先级污染物进行补救。因此，了解1，4-二氧六环衰减和生物修复能力对环境保护机构、场地所有者和公众都很重要。拟议的研究同时旨在确定微生物介导的1，4-二氧六环 ** 降解的生物标志物，并开发1，4-二氧六环降解富集培养物作为潜在的生物修复工具。这项工作将包括实地采样两个1，4-二氧六环污染的网站-南部安大略垃圾填埋场和一个 ** 前化工厂在得克萨斯州东南部。微生物生物量将从垃圾渗滤液和地下水中过滤出来。环境微生物将通过宏基因组测序进行表征，并培养1，4-二氧六环 ** 降解剂并表征其活性。将 ** 从采样点以及不同环境的公共宏基因组序列中鉴定参与1，4-二氧六环降解的基因，** 确定地球仪中1，4-二氧六环降解的流行率和潜力。准确的场地评估 ** 和获得具有成本效益的生物补救办法将为场地所有者带来巨大的经济利益。根据 ** 二氧六环的毒性和流行程度，预计 ** 加拿大环境部和美国环境保护署将在未来几年内实施最高污染物水平。因此，开发 ** 生物标志物以评估1，4-二氧六环降解的潜力和建立生物补救工具是 ** 确保加拿大决策者和场地所有者对 ** 新规定的最大污染物水平作出迅速、适当反应的积极步骤。