Investigating Toxicity of Airborne Metal Nanoparticles: Mechanisms and Characterization

纳米颗粒（NP，体外毒性测试系统，用于更确定地筛选、排序和预测纳米颗粒的毒性作用；ii）使用毒性基因组学方法识别和表征纳米颗粒毒性的因果因素。在接下来的五年里，我的短期目标是评估吸入性金属NPs的毒性，并通过提供更多关于其病因和机制的信息，对NPs进行更深入的动物研究。我特别建议：1)阐明NP增强肺部细菌感染的潜在原因和机制；2)评估肺部NP暴露对心血管系统的影响。******随着这项研究的成功完成，将有许多好处提供(1)HQP独特的培训机会，为一个新的科学分支（纳米毒理学）；(2)关于NP毒性等级和NP优先级的有价值信息，作为一种具有成本效益的筛选方法进行体内试验；(3)了解NP暴露产生生物反应的机制，因为这将使我们在制定安全NP设计策略方面处于有利地位。

Nanoparticle (NP, in vitro toxicity testing system to screen, rank and predict with greater certainty the toxic effects of NPs and ii) identify and characterize the causal factors and NP toxicity using a toxicogenomic approach. Over the next five years, my short-term objectives will assess the toxicity of inhaled metal NPs and prioritize NPs for more in depth in animal studies by providing more information on its causal factors and mechanisms. I specifically propose to: 1) elucidate the underlying causes and mechanisms by which NPs enhance lung bacterial infection and 2) assess the effects of pulmonary NP exposure on the cardiovascular system.******With successful completion of this research, there will be many benefits providing (1) HQP with unique training opportunities for a new branch of science (nanotoxicology); (2) valuable information on the rank of NP toxicity and prioritization of NPs for in vivo testing as a cost-effective screening; (3) understanding of the mechanisms by which NP exposures produce biological responses as this will put us in a strong position to develop strategies to design safe NPs.