Characterization of Toxicity of Airborne ENMs using Direct in Vitro Exposure

使用直接体外暴露表征空气中 ENM 的毒性

基本信息

批准号：
10081175
负责人：
Arantzazu Eiguren Fernandez
金额：
$ 48.72万
依托单位：
AEROSOL DYNAMICS, INC.
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-05-01 至 2022-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10081175
关键词：
Address Aerosols Air Analysis of Variance Area Asses Biological Biological Assay Biological Monitoring Breathing Carbon Nanotubes Cell Culture Techniques Cell Line Cell Survival Cells Cellular Stress Characteristics Chemicals Coculture Techniques Complex Copper Cosmetics Cultured Cells Data Reporting Deposition Development Devices Dimensions Disease Dose Drug Delivery Systems Electronics Engineering Epithelial Cells Exposure to Health Human Immune In Situ In Vitro Individual Industry Inhalation Device Injury Knowledge Laboratories Lead Length Life Liquid substance Lung Measurement Measures Methods Monitor Occupational Oxides Particle Size Performance Phase Physiological Policies Property Reproducibility Risk Safety Sampling Semiconductors Small Business Innovation Research Grant Spottings Statistical Data Interpretation Structure Sunscreening Agents Surface System Technology Testing Time Toxic effect Toxicology Uncertainty United States National Institutes of Health Welding Workplace aerosolized biological systems chemical property community setting consumer product cost experimental study improved in vitro Assay inflammatory marker instrument man monolayer nanomaterials nanoparticle novel novel strategies particle physical property portability prototype response sensor tool toxicant

项目摘要

Project Summary In the last decade, the use of engineered nanomaterials (NMs) (man-made materials with at least one dimension < 100 nm) has exponentially increased with their incorporation into new or developing technologies. More industries are using these NMs as components of sunscreens and cosmetics, drug delivery systems, structural materials, semiconductors, and sensors, as a result of their enhanced properties (strength, stiffness, electrical characteristics, and chemical and biological reactivity). However, these beneficial characteristics may also confer other detrimental properties once in contact with biological systems. Even though the number of NMs entering the market is increasing every year, there is a lack of information regarding the degree and conditions in which workers and consumers are exposed to these nanomaterials. Needed is a means to rapidly and reliably assess the toxicological properties of the increasing number of NMs, and to do so in a manner that can provide physiologically relevant toxicological information. Our project addresses this need with a novel approach for the direct exposure of air-liquid interface (ALI) cell cultures, both as monolayer and more complex cultures, to airborne NMs. Our approach is shown to be efficient and reproducible, providing results in minimal exposure times, even under environmentally realistic conditions. This project will carry this approach forward to provide a robust, versatile and compact device for exposing ALI cell cultures to aerosolized NMs. Together with rapid development of new methods and assays to cheaply and rapidly asses the biological effects of toxicants, our device will provide a new tool to study the mechanisms by which exposure to airborne NMs may lead to injury and/or disease, and improve our ability to examine and predict possible mechanisms of action of NMs.

项目摘要在过去的十年中，使用工程纳米材料（NMS）（人造材料至少有一个）尺寸<100 nm）随着将其纳入新技术或发展技术的成倍增加。越来越多的行业将这些NMS作为防晒和化妆品的组成部分，药物输送系统，结构材料，半导体和传感器，由于其性能增强（强度，刚度，电特性以及化学和生物反应性）。但是，这些有益特征可能一旦与生物系统接触，也赋予其他有害特性。即使数量进入市场的NMS每年都在增加，缺乏有关学位和工人和消费者暴露于这些纳米材料的条件。所需的一种方法可以快速可靠地评估越来越多的毒理学特性 NMS，并以可以提供生理相关的毒理学信息的方式这样做。我们的项目通过一种新的方法来解决这种需求，以直接暴露空气界面（ALI）细胞培养作为单层和更复杂的文化，对机载NMS。我们的方法被证明是有效的，可再现的，即使在环境现实的条件下，也可以提供最小的暴露时间。这项目将采用这种方法，以提供一个可靠的，多功能和紧凑的设备，用于暴露Ali Cell 培养为雾化的NM。以及快速开发新方法和廉价的测定法快速评估有毒物质的生物学作用，我们的设备将提供一种新工具来研究机制哪些接触空气迁移的NM可能会导致伤害和/或疾病，并提高我们检查和/或预测NMS作用的可能机制。