Characterization of Toxicity of Airborne ENMs using Direct in Vitro Exposure

使用直接体外暴露表征空气中 ENM 的毒性

• 批准号: 10081175
• 负责人: Arantzazu Eiguren Fernandez
• 金额: $ 48.72万
• 依托单位: AEROSOL DYNAMICS, INC.
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-05-01 至 2022-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10081175
• 关键词: Address; Aerosols; Air; Analysis of Variance; Area; Asses; Biological; Biological Assay; Biological Monitoring; Breathing; Carbon Nanotubes; Cell Culture Techniques; Cell Line; Cell Survival; Cells; Cellular Stress; Characteristics; Chemicals; Coculture Techniques; Complex; Copper; Cosmetics; Cultured Cells; Data Reporting; Deposition; Development; Devices; Dimensions; Disease; Dose; Drug Delivery Systems; Electronics; Engineering; Epithelial Cells; Exposure to; Health; Human; Immune; In Situ; In Vitro; Individual; Industry; Inhalation Device; Injury; Knowledge; Laboratories; Lead; Length; Life; Liquid substance; Lung; Measurement; Measures; Methods; Monitor; Occupational; Oxides; Particle Size; Performance; Phase; Physiological; Policies; Property; Reproducibility; Risk; Safety; Sampling; Semiconductors; Small Business Innovation Research Grant; Spottings; Statistical Data Interpretation; Structure; Sunscreening Agents; Surface; System; Technology; Testing; Time; Toxic effect; Toxicology; Uncertainty; United States National Institutes of Health; Welding; Workplace; aerosolized; biological systems; chemical property; community setting; consumer product; cost; experimental study; improved; in vitro Assay; inflammatory marker; instrument; man; monolayer; nanomaterials; nanoparticle; novel; novel strategies; particle; physical property; portability; prototype; response; sensor; tool; toxicant

Project Summary In the last decade, the use of engineered nanomaterials (NMs) (man-made materials with at least one dimension < 100 nm) has exponentially increased with their incorporation into new or developing technologies. More industries are using these NMs as components of sunscreens and cosmetics, drug delivery systems, structural materials, semiconductors, and sensors, as a result of their enhanced properties (strength, stiffness, electrical characteristics, and chemical and biological reactivity). However, these beneficial characteristics may also confer other detrimental properties once in contact with biological systems. Even though the number of NMs entering the market is increasing every year, there is a lack of information regarding the degree and conditions in which workers and consumers are exposed to these nanomaterials. Needed is a means to rapidly and reliably assess the toxicological properties of the increasing number of NMs, and to do so in a manner that can provide physiologically relevant toxicological information. Our project addresses this need with a novel approach for the direct exposure of air-liquid interface (ALI) cell cultures, both as monolayer and more complex cultures, to airborne NMs. Our approach is shown to be efficient and reproducible, providing results in minimal exposure times, even under environmentally realistic conditions. This project will carry this approach forward to provide a robust, versatile and compact device for exposing ALI cell cultures to aerosolized NMs. Together with rapid development of new methods and assays to cheaply and rapidly asses the biological effects of toxicants, our device will provide a new tool to study the mechanisms by which exposure to airborne NMs may lead to injury and/or disease, and improve our ability to examine and predict possible mechanisms of action of NMs.

项目概要 在过去的十年中，工程纳米材料（NM）（至少含有一种的人造材料）的使用 随着它们融入新技术或开发中的技术，尺寸< 100 nm）呈指数级增长。 越来越多的行业正在使用这些纳米材料作为防晒霜和化妆品、药物输送系统的成分， 结构材料、半导体和传感器，由于其增强的性能（强度、刚度、 电气特性、化学和生物反应性）。然而，这些有益的特性可能 一旦与生物系统接触，还会产生其他有害特性。尽管数量 进入市场的 NM 每年都在增加，但缺乏关于其程度和程度的信息。 工人和消费者接触这些纳米材料的条件。 需要一种方法来快速可靠地评估越来越多的毒理学特性 NM，并以可以提供生理相关毒理学信息的方式进行。我们的项目 通过直接暴露气液界面 (ALI) 细胞培养物的新方法满足了这一需求， 作为单层和更复杂的培养物，到机载 NM。我们的方法被证明是有效的并且 可重复，即使在现实的环境条件下，也能在最短的曝光时间内提供结果。这 项目将继续推进这一方法，提供一种坚固、多功能且紧凑的设备，用于暴露 ALI 细胞 培养物雾化 NM。随着新方法和检测方法的快速发展， 快速评估有毒物质的生物效应，我们的设备将提供一种新工具来研究其机制 暴露于空气中的 NM 可能会导致伤害和/或疾病，并提高我们检查和治疗的能力 预测 NM 可能的作用机制。