No need for beads! Virus-derived biological standards for nanoscale flow cytometry

不需要珠子！

• 批准号: 531917-2018
• 负责人: Langlois, MarcAndré
• 金额: $ 9.02万
• 依托单位: University of Ottawa
• 依托单位国家: 加拿大
• 项目类别: Idea to Innovation
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=656597
• 关键词: No; need; beads; Virus; derived

Nanoscale flow cytometry (NFC) is an emerging and transformative technology that is designed to analyze the composition of cell-derived surface markers expressed on minute biological particles, most notably viruses and extracellular vesicles (EVs). EVs resemble tiny bubbles constantly released by all cell types and serve as a means of communication to coordinate growth, differentiation, but also to warn other cells of potential dangers such as injury, infection and diseases such as cancer, Parkinson's and Alzheimer's. Analyzing the surface markers of EVs isolated from biological fluids such as blood, saliva and urine may provide early warning signs of disease or infection. As such, markers on the surface of EVs are poised to become valuable and powerful tools for medical and veterinary clinical diagnostics. Despite NFC being capable of analyzing EV surface markers, its broad deployment and adoption by hospitals and research centers has been hindered by the absence of suitable commercial instrument calibration standards and experimental controls. We have developed fluorescent virus-derived particles that mimic the size and physical properties of EVs, and thereby act as extremely reliable and effective controls for NFC. The viruses can be produced consistently, cheaply and in large quantities. The work proposed here will further improve these viruses to meet specific research and clinical requirements. The anticipated outcome is the production of 10 different virus-derived standards or controls that can be used in various research and clinical NFC applications for the accurate analysis of EV surface markers. We will ensure the supply of theses virus-derived particles through the creation of a start-up company dedicated to making them. The start-up will supply NFC instrument manufacturers and reagent distributors with the virus-derived standards, and they will make them available to their academic and industry clients. Economic and societal benefits to Canada include the training of highly qualified personnel, and the creation of a start-up that will manufacture a reagent for NFC applications which will, in turn, lead to a new generation of non-invasive diagnostic tests for disease and infection for use in humans, fish and livestock.**************

纳米级流式细胞术 (NFC) 是一项新兴的变革性技术，旨在分析微小生物颗粒（尤其是病毒和细胞外囊泡 (EV)）上表达的细胞源性表面标记物的组成。 EV 类似于所有细胞类型不断释放的微小气泡，作为协调生长、分化的通讯手段，同时也警告其他细胞潜在的危险，如受伤、感染和癌症、帕金森氏症和阿尔茨海默氏症等疾病。分析从血液、唾液和尿液等生物体液中分离出的 EV 的表面标记，可以提供疾病或感染的早期预警信号。因此，电动汽车表面的标记有望成为医疗和兽医临床诊断有价值且强大的工具。尽管 NFC 能够分析电动汽车表面标记，但由于缺乏合适的商业仪器校准标准和实验控制，医院和研究中心的广泛部署和采用受到了阻碍。我们开发了源自​​荧光病毒的颗粒，可以模仿电动汽车的大小和物理特性，从而作为 NFC 极其可靠和有效的控制。这些病毒可以持续、廉价、大量地生产。这里提出的工作将进一步改进这些病毒，以满足特定的研究和临床要求。预期成果是生产 10 种不同的病毒衍生标准品或对照品，可用于各种研究和临床 NFC 应用，以准确分析 EV 表面标记。我们将通过创建一家专门制造这些病毒颗粒的初创公司来确保这些病毒颗粒的供应。该初创公司将为 NFC 仪器制造商和试剂分销商提供病毒衍生标准，并将其提供给学术和行业客户。加拿大的经济和社会效益包括培训高素质人员，以及创建一家初创公司，生产用于 NFC 应用的试剂，从而开发出用于人类、鱼类和牲畜的新一代疾病和感染非侵入性诊断测试。 **************