The Need for Speed: Understanding the Importance of Different ELF3 Nuclear Localisation Mechanisms
对速度的需求:了解不同 ELF3 核定位机制的重要性
基本信息
- 批准号:BB/Z514998/1
- 负责人:
- 金额:$ 52.82万
- 依托单位:
- 依托单位国家:英国
- 项目类别:Fellowship
- 财政年份:2024
- 资助国家:英国
- 起止时间:2024 至 无数据
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
Circadian clocks are endogenous timekeeping mechanisms that enable organisms to coordinate their internal physiological responses with the external environmental. Manipulation of the plant circadian clock has been critical for plant domestication, while further alterations to clock function will contribute to the generation of crop varieties suitable for the changing climate. Over the last three decades of plant circadian research, we have developed detailed genetic and mathematical models of the clock system. In contrast, studies investigating the protein dynamics of the plant circadian clock are nascent. Where and when circadian proteins are active in the cell remains largely unknown. Understanding the circadian clock from a protein perspective is necessary to fully conceptualise and understand the impact of gene mutations on function and phenotype.The research proposed in this fellowship will investigate the cellular dynamics of the plant circadian protein EARLY FLOWERING3 (ELF3), a key hub protein within the plant circadian clock. So far, all functions of ELF3 are dependent on its localisation to the nucleus. However, it remains unknown how ELF3 arrives in the nucleus. Originally, ELF3 was proposed to localise to the nucleus through a specific sequence motif. However, this sequence motif is present in only a small number of plant species. Instead, my research suggests that ELF3 localises to the nucleus through interactions with other proteins. Understanding how these protein-protein interactions mediates the nuclear localisation of ELF3 is critical in understanding how ELF3 functions.The proposed work will utilise cellular, biochemical, and genetic approaches to provide new fundamental insights into how ELF3 localises to the nucleus and the contribution of this mechanism for its overall functionality.
生物钟是一种内源性的计时机制,使生物体能够协调其内部生理反应与外部环境。植物生物钟的调控对于植物驯化至关重要,而生物钟功能的进一步改变将有助于产生适合气候变化的作物品种。在过去三十年的植物昼夜节律研究中,我们已经开发了详细的生物钟系统的遗传和数学模型。相比之下,研究植物生物钟的蛋白质动力学才刚刚起步。昼夜节律蛋白在细胞中何时何地活跃仍然是未知的。从蛋白质的角度理解生物钟是必要的,以充分概念化和理解基因突变对功能和表型的影响。这项研究计划将研究植物生物钟蛋白EARLY FLOWERING 3(ELF 3)的细胞动力学,ELF 3是植物生物钟中的关键枢纽蛋白。到目前为止,ELF 3的所有功能都依赖于其在细胞核中的定位。然而,ELF 3如何到达细胞核仍然是未知的。最初,ELF 3被提出通过特定的序列基序定位于细胞核。然而,这种序列基序仅存在于少数植物物种中。相反,我的研究表明,ELF 3通过与其他蛋白质的相互作用定位于细胞核。了解这些蛋白质-蛋白质相互作用如何介导ELF 3的核定位对于了解ELF 3如何发挥功能至关重要。拟议的工作将利用细胞,生物化学和遗传学方法,为ELF 3如何定位于细胞核以及该机制对其整体功能的贡献提供新的基本见解。
项目成果
期刊论文数量(0)
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James Ronald其他文献
Ultrasound-guided non-targeted liver core biopsy: comparison of the efficacy of two different core needle biopsy systems using an ex-vivo animal model and retrospective review of clinical experience
- DOI:
10.1016/j.clinimag.2020.01.005 - 发表时间:
2020-05-01 - 期刊:
- 影响因子:
- 作者:
Lisa M. Ho;Avani A. Pendse;James Ronald;Marcus Luciano;Daniele Marin;Tracy A. Jaffe;Rendon C. Nelson - 通讯作者:
Rendon C. Nelson
Length of Stay Predicts Risk of Early Infection for Hospitalized Patients Undergoing Central Venous Port Placement
- DOI:
10.1016/j.jvir.2019.10.017 - 发表时间:
2020-03-01 - 期刊:
- 影响因子:
- 作者:
Linnan Tang;Charles Y. Kim;Jonathan G. Martin;Waleska M. Pabon-Ramos;Alan A. Sag;Paul V. Suhocki;Tony P. Smith;James Ronald - 通讯作者:
James Ronald
Percutaneous Gastrojejunostomy Tube Insertion in Patients with Surgical Gastrojejunal Anastomoses: Analysis of Success Rates and Durability
- DOI:
10.1016/j.jvir.2020.10.001 - 发表时间:
2021-02-01 - 期刊:
- 影响因子:
- 作者:
Christopher J.R. Gallo;Andre M. Agassi;David Y. Johnson;James Ronald;Waleska M. Pabon-Ramos;Alan A. Sag;Jonathan G. Martin;Paul V. Suhocki;Tony P. Smith;Dan G. Blazer;Charles Y. Kim - 通讯作者:
Charles Y. Kim
Percutaneous gastrojejunostomy tubes: Identification of predictors of retrograde jejunal limb migration into the stomach
- DOI:
10.1016/j.clinimag.2020.10.036 - 发表时间:
2021-02-01 - 期刊:
- 影响因子:
- 作者:
David Y. Johnson;Christopher J.R. Gallo;Andre M. Agassi;Alan A. Sag;Jonathan G. Martin;Waleska Pabon-Ramos;James Ronald;Paul V. Suhocki;Tony P. Smith;Charles Y. Kim - 通讯作者:
Charles Y. Kim
Intravascular Ultrasound Guidance for Transjugular Intrahepatic Portosystemic Shunt Creation Reduces Laboratory Markers of Acute Liver Injury
- DOI:
10.1016/j.jvir.2023.06.034 - 发表时间:
2023-10-01 - 期刊:
- 影响因子:
- 作者:
Vikram F. Gupta;Andre Agassi;Jonathan G. Martin;Brendan C. Cline;Charles Y. Kim;James Ronald - 通讯作者:
James Ronald
James Ronald的其他文献
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