Mass Transfer Coefficient Measurement in Single-use Bioreactor - Application of the Endoscopy Technique

对以更高规模和更低成本提供疫苗存在巨大需求。目前，经济上最优化的解决方案似乎是采用更大尺寸的一次性生物处理系统。这导致降低 ** 现有疫苗的生产成本，并最终促进新疫苗的开发。赛诺菲巴斯德拥有最广泛的疫苗，保护人口免受26种 ** 传染病中的20种。赛诺菲是加拿大最大的疫苗生产公司之一，其疫苗部门 ** 对利用一次性充气搅拌生物反应器以工业规模生产疫苗感兴趣。**尽管通常使用的曝气搅拌生物反应器，知识的各种过程 ** 变量的性能上的一次性生物处理系统的影响仍然是有限的。许多行业中使用的充气 ** 搅拌生物反应器的性能高度依赖于将氧气从 ** 气相转移到反应器内的液相的能力。理解氧气传质的一个关键部分是 ** 与能够确定生物反应器中气泡的大小有关。因此，** 拟议研究项目的目标是研究生物反应器中气/液相互作用的流体动力学，作为设计参数和操作条件的 ** 函数，并开发一种最佳设计，以获得 ** 有效的氧气传质速率，明确的体积传质系数，这是通气搅拌生物反应器内疫苗生产所需的。为了实现这些目标，采用了广泛的实验 **，包括先进的可视化和图像分析技术，如内窥镜（EnviroCam）和光学 ** 溶解氧仪（DO）。拟议项目的结果将使加拿大生物加工公司和疫苗 ** 制造商能够在其生产系统中应用一次性生物反应器。这将提高 ** 产品质量，有效利用电力，提高生产能力，并节省资本/运营成本。

There is an enormous demand to provide vaccines at higher scales and lower costs. Currently the economically**optimized solution seems to employ larger size single-use bioprocessing systems. This results in lowering**existing vaccines production cost and ultimately facilitating the development of new vaccines.**Sanofi Pasteur has the broadest available range of vaccines, protecting population against 20 out of 26**infectious diseases. The vaccines division of Sanofi, one of Canada's largest vaccine producing company is**interested in utilizing a single-use aerated stirred bioreactor for the production of vaccines at an industrial scale.**Despite the common use of the aerated stirred bioreactors, knowledge about the influence of various process**variables on performance of the single-use bioprocessing system is still limited. The performance of the aerated**stirred bioreactors utilized in numerous industries is highly dependent on the ability to transfer oxygen from the**gas phase to the liquid phase inside the reactors. A key part of understanding the mass transfer of oxygen is**related to being able to determine the size of the gas bubbles in the bioreactors. Therefore the goals in the**proposed research project are to investigate the hydrodynamics of gas/liquid interaction in the bioreactors as a**function of the design parameters and operating conditions and to develop an optimal design in obtaining an**efficient oxygen mass transfer rate, explicitly the volumetric mass-transfer coefficient, required for the vaccine**production inside of the aerated stirred bioreactor. To achieve these objectives, extensive experimentations**including advanced visualization and image analysis technique such as endoscopy (EnviroCam), and optical**dissolved oxygen meter (DO) are employed.**Results from the proposed project will enable the Canadian bioprocessing companies and vaccine**manufacturers to apply the single-use bioreactors in their production systems. This will lead to an enhanced**product quality, efficient use of power, increased production capacity, and capital/operational cost savings.