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Development and fundamentals of membrane processes

膜工艺的发展和基础

基本信息

批准号：
RGPIN-2015-04479
负责人：
Moresoli, Christine
金额：
$ 2.55万
依托单位：
University of Waterloo
依托单位国家：
加拿大
项目类别：
Discovery Grants Program - Individual
财政年份：
2018
资助国家：
加拿大
起止时间：
2018-01-01 至 2019-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=660187
关键词：
Development fundamentals membrane processes

项目摘要

Advances in technologies for the purification of therapeutic protein products are needed. Current technologies can represent up to 80% of the total manufacturing costs. Alternative protein purification processes should be able to deliver high purity products at affordable costs.******The long term objective of this research program is to develop selective and cost efficient protein purification processes. Promising purification process alternatives consist of membrane chromatography materials which offer high selectivity and throughput by engineering their structure and developing materials responsive to processing conditions. ***Efficient membrane chromatography purification processes will be obtained by extensive characterization of the material properties and by correlating these material properties to specific characteristics of therapeutic proteins. Our work will extend beyond the traditional experimental approach by incorporating novel mathematical models that will capture the dynamic nature of the materials. We will also develop novel protein purification membrane materials based on environmentally friendly processes. Sophisticated material characterization techniques will be employed to obtain microscopic and transient characteristics of the purification process. ***Long term impact of these alternative protein purification processes will eventually convert to high quality therapeutic protein products being available to the Canadian populations such as for cancer diagnostics and treatments.***This program will provide training to twenty four (24) highly qualified personnel (HQP) who will develop and gain hands-on skills in protein and material characterization, protein purification process development as well as data analysis and mathematical tools. In addition to these skills, HQP will acquire strong communication and training skills, will be exposed to multi-disciplinary and collaborative research environment and benefit from international expertise in protein expression and state-of-the art facilities. Trained HQP will be strategically positioned to join the industrial or academic sector and become leading experts in protein purification operations and material development.*****

需要在纯化治疗性蛋白产品的技术方面取得进展。目前的技术可以占到总制造成本的80%。替代的蛋白质纯化工艺应该能够以可承受的成本提供高纯度的产品。******这项研究计划的长期目标是开发选择性和成本效益高的蛋白质纯化工艺。膜层析材料是一种很有前途的纯化方法，通过设计其结构和开发对处理条件有响应的材料，可以提供高选择性和高通量。***通过对材料特性的广泛表征以及将这些材料特性与治疗蛋白的特定特性相关联，将获得高效的膜层析纯化过程。我们的工作将超越传统的实验方法，通过结合新颖的数学模型来捕捉材料的动态特性。我们还将开发基于环保工艺的新型蛋白质纯化膜材料。将采用复杂的材料表征技术来获得净化过程的微观和瞬态特征。***这些替代蛋白质纯化过程的长期影响最终将转化为加拿大人群可用的高质量治疗性蛋白质产品，例如用于癌症诊断和治疗。***该项目将为24名高素质人员（HQP）提供培训，他们将发展并获得蛋白质和材料表征、蛋白质纯化工艺开发以及数据分析和数学工具方面的实践技能。除了这些技能外，HQP还将获得强大的沟通和培训技能，将接触多学科和合作研究环境，并受益于蛋白质表达方面的国际专业知识和最先进的设施。训练有素的HQP将战略性地加入工业或学术部门，成为蛋白质纯化操作和材料开发的领先专家。*****