DNA Aptamers for the Recovery and Identification Cryptosporidium parvum oocysts in Liquid Municipal Wastes

用于回收和鉴定液体城市废物中小隐孢子虫卵囊的 DNA 适体

• 批准号: 530924-2018
• 负责人: Ormeci, Banu
• 金额: $ 1.82万
• 依托单位: Carleton University
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=661250
• 关键词: DNA; Aptamers; Recovery; Identification; Cryptosporidium

Cryptosporidium parvum is an intestinal protozoan parasite that can cause severe diarrhea in humans and many**animals, release large numbers of oocysts (eggs) in the feces of those infected which then can contaminate soil,**liquid and solid wastes. Cryptosporidium has caused many outbreaks of gastroenteritis. In many cases**wastewater effluent is discharged into source waters used for drinking water, recreation and agriculture. Due to**the human health risk, it has been recommended by Health Canada to routinely monitor drinking water treatment**plant source waters for oocyst concentrations. However, routine methods for concentration and detection of the**oocysts with immunofluorescence microscopy have low or variable recovery rates, are time consuming and**require highly skilled lab personnel. This research will help in the development of a sensitive aptamer-based**electrochemical biosensor method which can detect and quantify C. parvum in samples of liquid municipal wastes**and can help to mitigate pathogen discharges into the water environment.

隐孢子虫是一种肠道原生动物寄生虫，可引起人类和许多 ** 动物严重腹泻，在感染者的粪便中释放大量卵囊（卵），然后污染土壤，** 液体和固体废物。隐孢子虫引起了许多胃肠炎的爆发。在许多情况下，** 废水排放到饮用水、娱乐和农业用水的源沃茨。由于 ** 对人类健康的风险，加拿大卫生部建议定期监测饮用水处理 ** 厂水源沃茨中的卵囊浓度。然而，常规的浓缩和免疫荧光显微镜检测卵囊的方法回收率低或可变，耗时且需要高度熟练的实验室人员。本研究将有助于开发一种灵敏的基于适体的电化学生物传感器方法，用于检测和定量C。在城市液体废物样本中，** 可以减少病原体排放到水环境中。