Development of a high-resolution technique to monitor landfarm hydrocarbon biodegradative activity

人类工业活动产生的碳氢化合物污染物，如重工业油，对大多数有机体都是有毒的。值得庆幸的是，土壤中的微生物群落含有细菌和真菌，它们能产生特殊的酶来降解这些复杂的碳氢化合物。“土地农场”是一种经过管理的小块土地，专门用于 ** 利用微生物活动，快速、安全地降解工业活动 ** 产生的碳氢化合物。里贾纳SK的EVRAZ需要一种廉价、可靠和高分辨率的技术来监测 ** 他们农场的生物降解活性。这将为管理实践提供信息，并使EVRAZ能够 ** 有效地促进微生物生长，以加快修复速度。此外，一个运作良好的 ** 土地农场为EVRAZ提供了修复其他公司产生的废物的能力。里贾纳大学的研究人员正在与EVRAZ公司合作。NA加拿大应用统计模型，以确定 ** 土地管理中对生物修复能力产生积极或消极影响的变量。将使用基因技术监测土地农场土壤中的生物活性 **，对 ** 生物降解酶的产量进行量化。重要的是，取样是按照严格的统计实验设计进行的，以 ** 确保可以检测到酶表达中具有生物意义的变化，并将其与土壤 ** 管理做法联系起来。此外，尖端遗传技术的使用将提供前所未有的深入了解土地农场中的微生物活动，使大学科学家能够识别关键的生物修复微生物，从而将诊断技术用于土地农场功能的廉价和快速读数。

Hydrocarbon contaminants, like heavy industrial oils, from human industrial activities are toxic to most**organisms. Thankfully microbial communities in soils contain bacterial and fungal members that produce**special enzymes to degrade these complex hydrocarbons. A "landfarm" is a managed plot of land used**specifically to exploit microbial activity for the rapid and safe degradation of hydrocarbons from industrial**activities. EVRAZ in Regina SK, requires an inexpensive, reliable, and high-resolution technique to monitor**the biodegradative activity in their landfarm. This will inform management practices and allow EVRAZ to**effectively promote microbial growth to accelerate the rates of remediation. Moreover, a well-functioning**landfarm provides EVRAZ with the capacity to remediate waste generated by other companies. Researchers at**the University of Regina are partnering with EVRAZ Inc. NA Canada to apply statistical modelling to identify**variables in land management that positively or negatively impact bioremediation capacity. Biological activity**in the landfarm soils will be monitored using genetic technologies that quantify the production of**biodegradative enzymes. Importantly, sampling has been designed with strict statistical experimental design to**ensure that biologically meaningful changes in enzyme expression can be detected and correlated to soil**management practices. Additionally, the use of cutting-edge genetic techniques will provide unprecedented**depth of insight into the microbial activities in the landfarm, allowing the university scientists to identify the**keystone bioremediating microbes and thus target diagnostic techniques for an inexpensive and rapid readout**of landfarm function.