Development of an Inorganic-Organic Nano-Assembly Bioimaging Platform

无机-有机纳米组装生物成像平台的开发

• 批准号: 543547-2019
• 负责人: Hemmer, Eva
• 金额: $ 1.82万
• 依托单位: University of Ottawa
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=680490
• 关键词: Development; Inorganic; Organic; Nano; Assembly

The development of nanobiotechnology for diagnosis and treatment of diseases addressing current health-related challenges is of great interest for our society. A better understanding of nano-bio-interactions is of paramount importance to design innovative and more efficient solutions for applications such as drug delivery or imaging. Mirexus Biotechnologies Inc (MBI) has developed plant-derived carbohydrate nanoparticles (Phytoglycogen, PhG, derived from sweet corn), so-called NanoDendrix(TM). These are applied in immunomodulation, drug delivery and as anti-infectives. The surface chemistry of these nanoparticles allows for their easy modification to endow the organic nanoparticles with additional functionalities, for instance luminescence due to organic dyes. However, the combination of NanoDendrix(TM) with inorganic nanoparticles has yet to be explored. Of particular interest are lanthanide-based nanoparticles (Ln-NPs), due to their outstanding optical properties based on the use of near-infrared light that is able to penetrate deeper into biological tissues and induces less scattering, phototoxicity, and photobleaching than commonly required UV-vis light. Therefore, this project will develop inorganic-organic nano-assemblies based on NanoDentrix(TM), whose surface will be decorated with sub-10nm sized lanthanide-based nanoparticles. This endows the NanoDentrix(TM) with near-infrared-based optical properties that will be used in order to gain deeper insight into their nano-bio-interaction and ultimately biodistribution. In order to achieve this, we will use the innovative technique of hyperspectral imaging providing environment-specific (e.g. biological cells) optical information. In turn, the knowledge gained will be leveraged for the design and development of more efficient nanoprobes for biomedical applications benefiting Canadian Society.

用于诊断和治疗疾病的纳米生物技术的发展，解决当前与健康相关的挑战，引起了我们社会的极大兴趣。更好地理解纳米生物相互作用对于为药物输送或成像等应用设计创新且更有效的解决方案至关重要。 Mirexus Biotechnologies Inc (MBI) 开发了植物源性碳水化合物纳米粒子（植物糖原，PhG，源自甜玉米），即所谓的 NanoDendrix(TM)。它们应用于免疫调节、药物输送和抗感染。这些纳米颗粒的表面化学性质使其易于修饰，从而赋予有机纳米颗粒额外的功能，例如有机染料的发光作用。然而，NanoDendrix(TM) 与无机纳米粒子的结合仍有待探索。特别令人感兴趣的是基于镧系元素的纳米粒子（Ln-NP），因为它们具有基于近红外光的出色光学特性，能够更深入地渗透到生物组织中，并且比通常所需的紫外-可见光产生更少的散射、光毒性和光漂白。因此，该项目将开发基于NanoDentrix(TM)的无机-有机纳米组件，其表面将用亚10纳米尺寸的镧系纳米粒子装饰。这赋予 NanoDentrix(TM) 近红外光学特性，可用于更深入地了解其纳米生物相互作用和最终的生物分布。为了实现这一目标，我们将使用高光谱成像创新技术提供特定环境（例如生物细胞）的光学信息。反过来，所获得的知识将用于设计和开发更有效的纳米探针，用于生物医学应用，造福加拿大社会。