Identifying and quantifying metabolites of a novel Metarhizium robersil strain

昆特伦理工大学可持续园艺研究所(ISH)将利用其在昆虫病原真菌方面的经验，与作物保护者有限公司合作，开发鉴定和量化一种新的罗伯茨绿僵菌(CPD006)产生的化学代谢物的方法和质量控制协议，该公司正在加拿大作为一种新的生物杀虫剂开发临时商标NoVil。加拿大没有鉴定和量化昆虫病原真菌次生代谢物的专门知识。一般来说，包括罗伯茨绿僵菌在内的昆虫病原真菌产生的生物活性物质种类繁多，大多是次生代谢产物，参与害虫的生物防治和土壤微生物群的相互作用。然而，有些是潜在的已知毒素或真菌毒素，监管当局对这些毒素在环境中的命运以及在大规模生产过程中工人暴露在环境中的问题表示关切。ISH实验室正在开发类似的方案，以识别和量化木霉真菌作为新的生物杀菌剂的非挥发性和挥发性次生代谢物。这将有助于作物保护者将一种有价值的新生物杀虫剂推向市场。该项目将提高匡特伦与私营公司合作开发基于真菌的天然环保作物保护产品的能力，实现我们支持可持续发展和绿色经济的目标，并将更多生物产品交到种植者和虫害管理人员手中。园艺专业的学生将从接触尖端环境产品中受益，他们将在园艺职业生涯中遇到和使用这些产品。

The Institute for Sustainable Horticulture (ISH) at Kwantlen Polytechnic University will bring its experience with entomopathogenic fungi to partner with Crop Defenders Ltd. to develop methods and quality controlprotocols for identification and quantification of chemical metabolites produced by a novel isolate ofMetarhizium robertsii (CPD006) under the provisional trade name, NoVil, the company is developing as a newbio-insecticide in Canada. The expertise to identify and quantify secondary metabolites of entomopathogenic fungi does not exist in Canada. Generally, entomopathogenic fungi including Metarhizium robertsii produce a wide variety of biologically active compounds, mostly as products of secondary metabolism, which areinvolved in biocontrol of insect pests and other soil microbiome interactions. Some however are potentially known toxins or mycotoxins and concerns have been raised by regulatory authorities regarding the fate of thesemetabolites in the environment as well as the exposure of workers during mass production. The ISH lab isdeveloping similar protocols to identify and quantify both non-volatile and volatile secondary metabolites of Trichoderma fungi as new biofungicides. This will assist Crop Defenders to bring a valuable newbio-insecticide to the market. The project will increase Kwantlen's capacity to partner with private companies developing natural, fungal-based environmentally protective crop protection products, achieving our goal of supporting sustainability and the green economy and putting more biological products into the hands ofgrowers and pest management personnel. Horticulture students will benefit by exposure to cutting edgeenvironmental products which they will encounter and use in their horticulture careers.