Quantitative PCR Assays to Detect Mycoplasma bovis and Ureaplasma diversum in Cattle

检测牛体内牛支原体和多样化脲原体的定量 PCR 检测

• 批准号: 538298-2018
• 负责人: Adams, Paul
• 金额: $ 1.82万
• 依托单位: Kwantlen Polytechnic University
• 依托单位国家: 加拿大
• 项目类别: Applied Research and Development Grants - Level 1
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=681078
• 关键词: Quantitative; PCR; Assays; Detect; Mycoplasma

The Kwantlen Polytechnic University (KPU) Genomics Lab will use its expertise to develop quantitative polymerase chain reaction (Q-PCR) tests for Mycoplasma bovis (M. bovis) and Ureaplasma diversum (U. diversum) in collaboration with L'Alliance Boviteq (Boviteq). Boviteq offers reproductive and genetic solutions to North American cattle producers from its world-class In-Vitro Fertilization (IVF) labs. These new genetic tools produced by the KPU Genomics Lab will enable Boviteq and their partners to effectively detect and monitor M. bovis and U. diversum in Canadian dairy and beef cattle for better reproductive success and product quality. M. bovis is the most common pathogen associated with several forms of clinical manifestations in cattle involving arthritis, pneumonia and mastitis. Critically, M. bovis are resistant to most traditional antibiotics and therefore dif?cult to treat and can spread quickly through a herd causing significant health decline and economic loss. U. diversum is a common inhabitant of the lower reproductive tract of cattle. Critically, U. diversum is associated with decreased conception rates and various maladies associated with reproductive failure. The Q-PCR tests developed by KPU experts will allow Boviteq and partners to detect M. bovis and U. diversum at early stages in their animals and allow them to deploy effective disease management strategies to reduce overall impact on animal health and economic loss. Students will benefit from the opportunity to participate in applied research and contribute to the sustainability of Canada's cattle industry. These new tools can be used immediately by Boviteq's many clients throughout North America to improve animal health and productivity.

昆特伦理工大学（KPU）基因组学实验室将利用其专业知识开发牛支原体（M。bovis）和分歧脲原体（U. Diversum）与L 'Alliance Boviteq（Boviteq）合作。Boviteq通过其世界一流的体外受精（IVF）实验室为北美牛生产商提供生殖和遗传解决方案。KPU基因组学实验室生产的这些新的遗传工具将使Boviteq及其合作伙伴能够有效地检测和监测M。bovis和U. diversum在加拿大奶牛和肉牛更好的繁殖成功率和产品质量。M.牛是最常见的病原体，与牛中涉及关节炎、肺炎和乳腺炎的几种形式的临床表现相关。关键是，M。牛对大多数传统抗生素具有耐药性，因此，它可以在牛群中迅速传播，造成显著的健康下降和经济损失。联合diversum是牛的下生殖道的常见居民。关键是，U。diversum与降低的受孕率和与生殖失败有关的各种疾病有关。KPU专家开发的Q-PCR检测将使Boviteq及其合作伙伴能够检测M。bovis和U.因此，他们可以在动物的早期阶段使用diversum，并使他们能够部署有效的疾病管理策略，以减少对动物健康的总体影响和经济损失。学生将受益于参与应用研究的机会，并为加拿大养牛业的可持续发展做出贡献。Boviteq在北美的许多客户可以立即使用这些新工具来改善动物健康和生产力。