Ultra-rapid full spectral detection optical system facilitating novel PCR based assays- The iPCR approach

超快速全光谱检测光学系统促进新型 PCR 检测 - iPCR 方法

• 批准号: 710573
• 金额: $ 12.74万
• 依托单位: B G RESEARCH LIMITED
• 依托单位国家: 英国
• 项目类别: GRD Proof of Concept
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=710573
• 关键词: Ultra; rapid; full; spectral; detection

This project will generate an optical detection system and associated software for true realtimedetermination of fluorescence levels generated during real-time PCR. BioGene (BG) hasa technology platform capable of completing the real-time PCR process in 10 minutes and thisspeed combined with independent reaction control lends itself to a number of novelapplications that could revolutionise the diagnostic field. “True real-time” PCR is a stepchange in this sector - the ability to perform reactions whereby the instrumentation can keepup with the chemistry and hence observe and collect data missed by the current one readingper cycle approach. The ability to monitor all visible wavelengths on a milliseconds timescalewhen combined with Ultra-Rapid PCR facilitates new assays centred on a machine learningapproach. Principally, the system can learn the ideal amplification characteristics of anyreaction, allowing processes such as factorial optimisation - the ability to optimise any newassay in a single run - by altering factors such as hold times and concentrations across a plate.This approach can be branded “intelligent PCR” (iPCR) as the system can monitor in cyclefluorescence as opposed to the whole reaction efficiency and so could automatically minimisereaction time, by detecting annealing, completion of extension and melting, and optimise eachreaction individually by altering hold times and temperatures in run. Benefits include makingcalls within 5 minutes and in cycle differential annealing and melt analyses. The majorcommercial benefit is the speed and facilitation of the machine learning approach, fordiagnostic users the ability to optimise any new assay in a single run is a very powerful tooland additionally makes much faster subsequent downstream diagnostic calls. Such a systemwill allow the use of novel assay types, supporting the medical community and generatinggrowth globally from UK government funded research.

该项目将产生一个光学检测系统和相关软件，用于实时PCR过程中产生的荧光水平的真正实时测定。BioGene（BG）的实时PCR技术平台能够在10分钟内完成实时PCR过程，这种速度与独立的反应控制相结合，可以为诊断领域带来革命性的新应用。“真正的实时”PCR是该领域的一个飞跃--能够执行反应，使仪器能够与化学反应保持同步，从而观察和收集当前每循环一次读取方法所遗漏的数据。当与Ultra-Rapid PCR结合时，在毫秒时间尺度上监测所有可见波长的能力促进了以机器学习方法为中心的新检测。原则上，该系统可以学习任何反应的理想放大特性，允许因子优化等过程-在单次运行中优化任何新测定的能力-通过改变因子，如保持时间和平板上的浓度。这种方法可以称为“智能PCR”（iPCR）由于该系统可以监测循环荧光而不是整个反应效率，因此可以通过检测退火，完成延伸和熔融，并通过改变运行中的保持时间和温度来单独优化每个反应。优点包括在5分钟内进行呼叫，并在循环中进行差异退火和熔体分析。主要的商业利益是机器学习方法的速度和便利性，对于诊断用户来说，在单次运行中优化任何新检测的能力是一个非常强大的工具，并且还可以更快地进行后续的下游诊断调用。这样的系统将允许使用新的检测类型，支持医学界，并从英国政府资助的研究中产生全球增长。