Receptor Tyrosine Kinase Transactivation in Neuronal Signalling, Stress, and Survival

受体酪氨酸激酶反式激活对神经元信号传导、应激和生存的影响

• 批准号: RGPIN-2019-04177
• 负责人: Beazely, Michael
• 金额: $ 2.33万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=684396
• 关键词: Receptor; Tyrosine; Kinase; Transactivation; Neuronal

Brain cells (neurons) communicate with one another using protein “receptors”. Major types of protein receptors include G protein-coupled receptors (GPCRs), receptor tyrosine kinases (RTKs), and ion channels such as the N-methyl-D-aspartate (NMDA) receptor. How the signalling of one type of receptor is influenced by the others (receptor cross-talk) is less understood. Our lab works to better understand a specific type of cross-talk called “RTK transactivation”, how it influences neuronal activity, its role in responding to neuronal stress, and promoting neuronal survival. This research is needed so we can fully understand the complexity of brain function in both healthy neurons, damaged neurons, or neurons experiencing stress.******Much of the work describing RTK transactivation research has been descriptive and carried out in non-neuronal models. One of our research objectives is to characterize RTK transactivation pathways in both neurons (primary cultures and ex vivo brain slice) and in novel neuronal-like cell lines such as the hippocampus-derived Ht22 cell line. In addition to answering several fundamental questions about RTK transactivation, developing these new model systems will be valuable to other researchers in neuroscience, including those interested in employing the Ht22 cell line as a hippocampal-like model.******Beyond the basic characterization of RTK transactivation, our second objective is to study the consequences of RTK transactivation on neuronal signalling, activity, and survival with a focus on NMDA receptor signalling and excitotoxicity (when NMDA receptors are over-activated). We have demonstrated that the 5-HT7 receptor is able to regulate NMDA receptor signalling and reduce excitotoxicity via platelet-derived growth factor (PDGF) receptor and TrkB receptor transactivation.******In several cells and tissues, including cardiac myocytes, vascular smooth muscle cells, pulmonary and renal epithelial cells, and in white blood cells, RTK signalling, and RTK transactivation specifically, is altered by stress or tissue damage. Our third objective is to determine the role of RTK transactivation in the neuronal response to stress. Does neuronal stress initiate RTK transactivation pathways as observed in other cell types? Are specific GPCR-RTK transactivation pathways disrupted in neuronal stress? Determining the scope of changes in RTK signalling and transactivation in neuronal stress will provide a more robust understanding of the cellular responses to stress in neurons.******As our research involves multiple techniques, model systems, and collaborations with other researchers, students in my lab will gain a diverse set of skills that will allow them to continue to advance knowledge in academic and industrial settings. The outcomes of our research will impact scientists and researchers across several disciplines, including basic and applied neuroscience, stress and stress neurobiology, as well as researchers in neuropsychiatry and neurology.

脑细胞（神经元）通过蛋白质“受体”相互交流。蛋白质受体的主要类型包括G蛋白偶联受体（GPCR）、受体酪氨酸激酶（RTK）和离子通道如N-甲基-D-天冬氨酸（NMDA）受体。一种受体的信号如何受到其他受体的影响（受体串扰）还不太清楚。我们的实验室致力于更好地了解一种称为“RTK反式激活”的特定类型的串扰，它如何影响神经元活动，它在响应神经元应激和促进神经元存活方面的作用。这项研究是必要的，这样我们就可以充分了解健康神经元、受损神经元或经历压力的神经元的大脑功能的复杂性。大部分描述RTK反式激活研究的工作都是描述性的，并在非神经元模型中进行。我们的研究目标之一是在神经元（原代培养物和离体脑切片）和新的神经元样细胞系，如海马衍生的Ht 22细胞系中表征RTK反式激活途径。除了回答关于RTK反式激活的几个基本问题外，开发这些新的模型系统对神经科学的其他研究人员也很有价值，包括那些有兴趣将Ht 22细胞系用作类海马模型的研究人员。除了RTK反式激活的基本特征之外，我们的第二个目标是研究RTK反式激活对神经元信号传导、活动和存活的后果，重点是NMDA受体信号传导和兴奋性毒性（当NMDA受体过度激活时）。我们已经证明，5-HT 7受体能够通过血小板衍生生长因子（PDGF）受体和TrkB受体反式激活调节NMDA受体信号传导并降低兴奋性毒性。在几种细胞和组织中，包括心肌细胞、血管平滑肌细胞、肺和肾上皮细胞，以及在白色血细胞中，RTK信号传导和RTK反式激活特别地被应激或组织损伤改变。我们的第三个目标是确定RTK反式激活在神经元应激反应中的作用。神经元应激是否启动RTK反式激活途径，如在其他细胞类型中观察到的那样？特异性GPCR-RTK反式激活通路在神经元应激中是否被破坏？确定神经元应激中RTK信号传导和反式激活的变化范围将提供对神经元中应激的细胞反应的更可靠的理解。由于我们的研究涉及多种技术，模型系统以及与其他研究人员的合作，我实验室的学生将获得各种技能，使他们能够继续在学术和工业环境中推进知识。我们的研究成果将影响多个学科的科学家和研究人员，包括基础和应用神经科学，压力和压力神经生物学，以及神经精神病学和神经病学的研究人员。