Reindeer Antler Velvet: a unique model to study cellular and molecular mechanisms underlying tissue regeneration

驯鹿鹿茸：研究组织再生的细胞和分子机制的独特模型

• 批准号: RGPIN-2017-04825
• 负责人: Biernaskie, Jeffrey
• 金额: $ 2.48万
• 依托单位: University of Calgary
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=685350
• 关键词: Reindeer; Antler; Velvet; unique; model

Mammalian evolution has selected for accelerated wound closure at the expense of generating fibrotic scar. To enable future development of therapies that improve skin healing, a comprehensive understanding of the molecular signals that promote a regenerative versus fibrotic healing response will be essential. Our current understanding of these divergent processes is poor, partly because we lack a robust mammalian model of tissue regeneration.******Deer antlers are the only mammalian organ capable of complete regeneration. Growing antlers are covered in skin (velvet) that contains hair follicles, glands, dense innervation and vascular supply. In exciting preliminary work, we discovered that after injury, antler velvet is capable of scarless regeneration, a phenomenon previously ascribed only to embryonic skin. Regenerated velvet wounds contain newly formed hair follicles, glands, dense innervation and pigmentation. In contrast, identical wounds in backskin (of the same animal) resulted in fibrotic scar, consisting of disorganized extracellular matrix and an absence of appendages, innervation or pigment. We will exploit this powerful comparative model to study the basic cellular and molecular dynamics that underlie these divergent modes of mammalian skin repair. We will test the hypothesis that regeneration is enabled by a unique gene expression program within velvet dermal fibroblasts.******Aim 1- To characterize the molecular regulators that enable skin regeneration versus formation of fibrotic scar. We will use RNAseq to perform a comparative transcriptomic analysis of injured antler velvet and backskin to identify the molecular mechanisms that enable velvet regeneration.******Aim 2 Functional evaluation of genes that are exclusively activated or repressed during velvet regeneration. Small molecules and lentiviral vectors designed to activate or inhibit identified genes or associated signaling pathways, will be applied to velvet and backskin wounds to ascertain their role in promoting regeneration or scar formation. ******Aim 3 - To isolate and characterize dermal progenitors from antler velvet. We hypothesize that velvet is enriched with dermal progenitors that enable regeneration. We will isolate progenitors from antler velvet or backskin dermis/mesenchyme and perform phenotypic characterization and transplantation experiments to compare their intrinsic functional capacities.******Aim 4 To characterize macrophage dynamics within resting antler velvet and during fibrotic skin repair versus regeneration. Macrophage are essential contributors to tissue regeneration. We hypothesize that macrophage exhibit a unique response to velvet injury which consequently modifies the dermal fibroblast repair program. Using immunostaining, cell sorting and cytometry we will document the composition of resident macrophages as well as the response to injury in backskin versus velvet.**

哺乳动物进化选择了加速伤口闭合，代价是产生纤维化瘢痕。为了使未来能够开发改善皮肤愈合的疗法，全面了解促进再生与纤维化愈合反应的分子信号将是必不可少的。我们目前对这些不同过程的理解很差，部分原因是我们缺乏一个强大的组织再生哺乳动物模型。鹿角是唯一能够完全再生的哺乳动物器官。生长中的鹿角被覆盖在含有毛囊、腺体、密集的神经支配和血管供应的皮肤（天鹅绒）中。在令人兴奋的初步工作中，我们发现，在受伤后，鹿茸能够无疤痕再生，这种现象以前只归因于胚胎皮肤。再生的天鹅绒伤口含有新形成的毛囊、腺体、密集的神经支配和色素沉着。相比之下，（同一动物）背部皮肤中的相同伤口导致纤维化瘢痕，由紊乱的细胞外基质组成，缺乏附属物、神经支配或色素。我们将利用这个强大的比较模型来研究哺乳动物皮肤修复这些不同模式的基础细胞和分子动力学。我们将测试这一假设，即再生是由天鹅绒真皮成纤维细胞内独特的基因表达程序实现的。目的1-表征使皮肤再生与纤维化瘢痕形成的分子调节剂。我们将使用RNAseq对受伤的鹿茸和背部皮肤进行比较转录组学分析，以确定使鹿茸再生的分子机制。目的2鹿茸再生过程中特异性激活或抑制基因的功能研究。 小分子和慢病毒载体旨在激活或抑制已识别的基因或相关的信号通路，将应用于天鹅绒和背部皮肤伤口，以确定它们在促进再生或疤痕形成中的作用。** 目的3 -从鹿茸中分离和表征真皮祖细胞。 我们假设天鹅绒富含能够再生的真皮祖细胞。我们将从鹿茸或背部皮肤真皮/间充质中分离祖细胞，并进行表型表征和移植实验，以比较它们内在的功能能力。目的4研究鹿茸中巨噬细胞的动态变化及其在纤维化皮肤修复与再生中的作用.巨噬细胞是组织再生的重要贡献者。我们假设巨噬细胞对天鹅绒损伤表现出独特的反应，从而改变了真皮成纤维细胞的修复程序。使用免疫染色，细胞分选和细胞计数，我们将记录驻留巨噬细胞的组成以及对背部皮肤与天鹅绒损伤的反应。