Establishing the essentiality of fats in the postnatal diet for optimal development
确定脂肪在产后饮食中对最佳发育的重要性
基本信息
- 批准号:RGPIN-2017-04746
- 负责人:
- 金额:$ 2.84万
- 依托单位:
- 依托单位国家:加拿大
- 项目类别:Discovery Grants Program - Individual
- 财政年份:2019
- 资助国家:加拿大
- 起止时间:2019-01-01 至 2020-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The overall goal of my NSERC program is to establish the essentiality of dietary fats for immunity in the early postnatal period. We will study 3 specific fats,,docosahexaenoic acid (DHA), arachidonic acid (AA) and phosphatidylcholine (PC), for which a supply is needed by the immune system, but they are not currently considered essential in the diet. The suckling period and the weaning period are both critical stages when the immune system matures and the animal develops oral tolerance (OT), the ability to distinguish between harmful and harmless oral antigens. We have demonstrated that adding DHA or PC to otherwise nutritionally adequate diets improves immune maturation and function. ******During the next 5y, my NSERC program will focus on 4 objectives:***1) Establishing the dietary essentiality of AA during immune development ***2) Optimizing dietary AA/DHA ratio for immune development ***3) Establishing the amount of PC in the diet for immune development, then using this PC to deliver/improve the supply of long chain polyunsaturated fatty acids (LCPUFA) to immune cells ***4) Identifying the change in plasma and immune phospholipids (PL) as a biomarker of intake******Using male and female Sprague-Dawley and allergen sensitive Brown Norway rats, we will study how changes in the AA, DHA/AA ratio and the PC content of the diet influence immune function during suckling and weaning. For the suckling studies we will change pups' intake through changing their dams' diets. For testing the essentiality of AA (below levels in breast milk) we will use an artificial rearing model. OT will be studied using methods that induce systemic or mucosal tolerance to the food protein ovalbumin (OVA). After inducing OT, plasma concentrations of OVA-specific IgE and IgG are measured. Immune cells are isolated from the spleen and characterized (by flow cytometry) then used to assess their ability to produce cytokines (Th1 or Th2 pattern) to normal physiological challenges' using mitogens or OVA. By measuring the change in plasma, breast milk, immune cell PL (content, fatty acid composition) and choline (content, composition) we propose to identify biomarkers that predict immune function and will provide targets for developing a novel PC supplement.******Hypothesizes: 1) AA is required; 2) a ratio of 1:2 (AA:DHA) will optimize immune maturation; 3) choline coming from free choline and PC (25:75) is optimal; and 4) the content and fatty acid composition of plasma PC will be an ideal biomarker to monitor LCPUFA status associated with optimal immune development.******This fundamental knowledge is needed to establish the dietary need of these lipids for immune development. Additionally, we will identify biomarkers that predict immune function and develop a PC supplement to provide all 3 lipids. My NSERC program will contribute to the unique training of different types of HPQ in the field of nutritional immunology.
我的NSERC计划的总体目标是建立饮食脂肪对出生后早期免疫力的重要性。 我们将研究3种特定的脂肪,二十二碳六烯酸(DHA),花生四烯酸(AA)和磷脂酰胆碱(PC),这些脂肪是免疫系统所需要的,但它们目前并不被认为是饮食中必不可少的。哺乳期和断奶期都是免疫系统成熟和动物产生口服耐受(OT)的关键阶段,即区分有害和无害口服抗原的能力。我们已经证明,在营养充足的饮食中添加DHA或PC可以提高免疫成熟和功能。** 在接下来的5年里,我的NSERC计划将重点关注4个目标:*1)确定AA在免疫发育期间的饮食必要性 *2)优化用于免疫发育的饮食AA/DHA比例 *3)确定用于免疫发育的饮食中PC的量,然后使用该PC向免疫细胞递送/改善长链多不饱和脂肪酸(LCPUFA)的供应 *4)鉴定血浆和免疫磷脂(PL)的变化作为摄入的生物标志物 ** 使用雄性和雌性Sprague-Dawley大鼠和过敏原敏感的Brown Norway大鼠,我们将研究AA,日粮中DHA/AA比例和PC含量影响哺乳期和断奶期的免疫功能。对于哺乳研究,我们将通过改变母鼠的饮食来改变幼鼠的摄入量。为了测试AA的必要性(低于母乳中的水平),我们将使用人工饲养模型。OT将使用诱导对食物蛋白卵清蛋白(OVA)的全身或粘膜耐受性的方法进行研究。诱导OT后,测量OVA特异性IgE和IgG的血浆浓度。从脾中分离免疫细胞并进行表征(通过流式细胞术),然后使用有丝分裂原或OVA评估其产生细胞因子(Th 1或Th 2模式)以应对正常生理挑战的能力。通过测量血浆,母乳,免疫细胞PL(含量,脂肪酸组成)和胆碱(含量,组成)的变化,我们提出鉴定预测免疫功能的生物标志物,并为开发新型PC补充剂提供目标。假设:1)需要AA; 2)1:2(AA:DHA)的比例将优化免疫成熟; 3)来自游离胆碱和PC(25:75)的胆碱是最佳的;以及4)血浆PC的含量和脂肪酸组成将是监测与最佳免疫发育相关的LCPUFA状态的理想生物标志物。需要这些基本知识来确定免疫发育对这些脂质的饮食需求。 此外,我们将确定预测免疫功能的生物标志物,并开发PC补充剂以提供所有3种脂质。我的NSERC项目将为营养免疫学领域不同类型的HPQ的独特培训做出贡献。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Field, Catherine其他文献
Comparing docosahexaenoic acid (DHA) concomitant with neoadjuvant chemotherapy versus neoadjuvant chemotherapy alone in the treatment of breast cancer (DHA WIN): protocol of a double-blind, phase II, randomised controlled trial
- DOI:
10.1136/bmjopen-2019-030502 - 发表时间:
2019-09-01 - 期刊:
- 影响因子:2.9
- 作者:
Newell, Marnie;Mackey, John R.;Field, Catherine - 通讯作者:
Field, Catherine
Associations between maternal folate status and choline intake during pregnancy and neurodevelopment at 3-4 years of age in the Alberta Pregnancy Outcomes and Nutrition (APrON) study.
- DOI:
10.1017/s2040174423000041 - 发表时间:
2023-06 - 期刊:
- 影响因子:1.7
- 作者:
Irvine, Nathalie;England-Mason, Gillian J.;Field, Catherine;Letourneau, Nicole C.;Bell, Rhonda F.;Giesbrecht, Gerald W.;Kinniburgh, David M.;MacDonald, Amy W.;Martin, Jonathan;Dewey, Deborah;APrON Study Team - 通讯作者:
APrON Study Team
Field, Catherine的其他文献
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{{ truncateString('Field, Catherine', 18)}}的其他基金
Establishing the essentiality of fats in the postnatal diet for optimal development
确定脂肪在产后饮食中对最佳发育的重要性
- 批准号:
RGPIN-2017-04746 - 财政年份:2021
- 资助金额:
$ 2.84万 - 项目类别:
Discovery Grants Program - Individual
Establishing the essentiality of fats in the postnatal diet for optimal development
确定脂肪在产后饮食中对最佳发育的重要性
- 批准号:
RGPIN-2017-04746 - 财政年份:2020
- 资助金额:
$ 2.84万 - 项目类别:
Discovery Grants Program - Individual
Upgrades to Multi-Lab Gas Chromatography System
升级至多实验室气相色谱系统
- 批准号:
RTI-2020-00116 - 财政年份:2019
- 资助金额:
$ 2.84万 - 项目类别:
Research Tools and Instruments
Establishing the essentiality of fats in the postnatal diet for optimal development
确定脂肪在产后饮食中对最佳发育的重要性
- 批准号:
RGPIN-2017-04746 - 财政年份:2018
- 资助金额:
$ 2.84万 - 项目类别:
Discovery Grants Program - Individual
Establishing the essentiality of fats in the postnatal diet for optimal development
确定脂肪在产后饮食中对最佳发育的重要性
- 批准号:
RGPIN-2017-04746 - 财政年份:2017
- 资助金额:
$ 2.84万 - 项目类别:
Discovery Grants Program - Individual
Programing of T cell function and development through the manipulation of dietary fat in the early postnatal period
通过控制产后早期的膳食脂肪来编程 T 细胞功能和发育
- 批准号:
RGPIN-2016-05649 - 财政年份:2016
- 资助金额:
$ 2.84万 - 项目类别:
Discovery Grants Program - Individual
Modulation of T cell function and development through the manipulation of diet-derived long chain polyunsaturated fatty acids
通过控制饮食来源的长链多不饱和脂肪酸来调节 T 细胞功能和发育
- 批准号:
121610-2011 - 财政年份:2015
- 资助金额:
$ 2.84万 - 项目类别:
Discovery Grants Program - Individual
Modulation of T cell function and development through the manipulation of diet-derived long chain polyunsaturated fatty acids
通过控制饮食来源的长链多不饱和脂肪酸来调节 T 细胞功能和发育
- 批准号:
121610-2011 - 财政年份:2014
- 资助金额:
$ 2.84万 - 项目类别:
Discovery Grants Program - Individual
Modulation of T cell function and development through the manipulation of diet-derived long chain polyunsaturated fatty acids
通过控制饮食来源的长链多不饱和脂肪酸来调节 T 细胞功能和发育
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$ 2.84万 - 项目类别:
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- 资助金额:
$ 2.84万 - 项目类别:
Research Tools and Instruments - Category 1 (<$150,000)
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