Investigation of different column packing methods on the efficiency of chromatographic separation for biotechnology and pharmaceutical applications

制备型液相色谱法是生物制药工业中纯化生物化合物的主要下游操作。填充床柱对色谱步骤的性能至关重要。随着新的柱填料方法的发展，有必要对柱进行资格鉴定，以确保它们以稳健和一致的方式执行。此外，随着新的分析程序和制备尺度方法的发展，需要改进的方法来填充柱或修改当前的方法是必需的。在过去的几年中，该应用程序的工业合作伙伴PolyAnalytik Inc.已经参与了制药和医疗设备行业的几个研究和开发项目（R&D）。十年来，PolyAnalytik公司也一直从事小分子和大分子分离色谱柱的销售。该组织在制药应用中使用的纯化和色谱技术方面获得了广泛的知识和经验，这使得该组织能够考虑开发独特的填充色谱柱，该色谱柱含有生物处理树脂，用于许多纯化应用（例如，用于蛋白质、肽、抗病毒等药物）。PolyAnalytik公司拥有几种柱填料的专有方法。在这项提案中，Bassi实验室将探索实验和数学建模工具，与Polyanalytik合作研究这些柱的填充柱效率，以进一步推进高效系统的开发。与UWO的Bassi实验室合作进行的柱填充效率调查研究对Polyanalytik至关重要，因为这种填充柱的发展将导致创新和独特的填充柱，用于高效色谱应用。

Preparative liquid chromatography is the dominant, downstream operation for the purification ofbiological compounds in the bio-pharmaceutical industries. The packed bed column is essential to the performance of the chromatographic step. As new column packing methods are developed, it is necessary to qualify columns to ensure they perform in a robust and consistent fashion. In addition, as new analytical procedures and preparative scale approaches are developed, improved methods are needed for column packing or modifications to current approaches are required. Over the past few years, the industrial partner in this application, PolyAnalytik Inc., has been a part of several research and development projects (R&D) in the pharmaceutical and medical device industries. PolyAnalytik Inc. has also been involved in selling chromatographic columns in the separation of small molecules and macromolecules for a decade. The extensive knowledge and experience gained by the organization in the purification and chromatographic techniques used in pharmaceuticals applications have allowed the organization to consider the development of uniquely packed chromatographic columns containing bioprocessing resins for many purification applications (e.g. for pharmaceuticals such as proteins, peptides, antiviruses). PolyAnalytik Inc. owns several proprietary approaches for column packing. In this proposal, the Bassi lab will explore experimental and mathematical modelling tools to investigate the packed column efficiency of these columns in collaboration with Polyanalytik to further advance the development of highly efficient systems. This investigative study on column packing efficiencies in partnership with the Bassi laboratory at UWO is crucial to Polyanalytik as the development of such packed columns as this will lead to innovative and distinctively packed columns for high efficiency chromatographic applications.