Investigation of different column packing methods on the efficiency of chromatographic separation for biotechnology and pharmaceutical applications

研究不同柱填充方法对生物技术和制药应用色谱分离效率的影响

• 批准号: 530871-2018
• 负责人: Bassi, Amarjeet
• 金额: $ 2.91万
• 依托单位: University of Western Ontario
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=687827
• 关键词: Investigation; different; column; packing; methods

Preparative liquid chromatography is the dominant, downstream operation for the purification ofbiological compounds in the bio-pharmaceutical industries. The packed bed column is essential to the performance of the chromatographic step. As new column packing methods are developed, it is necessary to qualify columns to ensure they perform in a robust and consistent fashion. In addition, as new analytical procedures and preparative scale approaches are developed, improved methods are needed for column packing or modifications to current approaches are required. Over the past few years, the industrial partner in this application, PolyAnalytik Inc., has been a part of several research and development projects (R&D) in the pharmaceutical and medical device industries. PolyAnalytik Inc. has also been involved in selling chromatographic columns in the separation of small molecules and macromolecules for a decade. The extensive knowledge and experience gained by the organization in the purification and chromatographic techniques used in pharmaceuticals applications have allowed the organization to consider the development of uniquely packed chromatographic columns containing bioprocessing resins for many purification applications (e.g. for pharmaceuticals such as proteins, peptides, antiviruses). PolyAnalytik Inc. owns several proprietary approaches for column packing. In this proposal, the Bassi lab will explore experimental and mathematical modelling tools to investigate the packed column efficiency of these columns in collaboration with Polyanalytik to further advance the development of highly efficient systems. This investigative study on column packing efficiencies in partnership with the Bassi laboratory at UWO is crucial to Polyanalytik as the development of such packed columns as this will lead to innovative and distinctively packed columns for high efficiency chromatographic applications.

在生物制药工业中，制备液相色谱是生物化合物纯化的主要下游操作。填充床柱对色谱柱的性能至关重要。随着新的柱填充方法的发展，有必要对柱进行鉴定，以确保它们以坚固和一致的方式运行。此外，随着新的分析方法和制备规模方法的发展，需要改进柱填充方法或对现有方法进行修改。在过去的几年里，这一应用的工业合作伙伴PolyAnalytik Inc.一直是制药和医疗器械行业的几个研发项目(R&D)的一部分。PolyAnalytik Inc.还参与销售分离小分子和大分子的色谱柱已有十年之久。该组织在制药应用中使用的纯化和色谱技术方面获得了广泛的知识和经验，这使得该组织能够考虑开发含有生物处理树脂的独特填充色谱柱，用于许多纯化应用(例如，用于蛋白质、多肽、抗病毒等药品)。PolyAnalytik Inc.拥有几种专有的柱填充方法。在这项提案中，BASSI实验室将探索实验和数学建模工具，与Polyanalytik合作研究这些柱的填充柱效率，以进一步推动高效系统的开发。这项与UWO的BASSI实验室合作进行的关于柱填充效率的调查研究对Polyanalytik至关重要，因为这种填充柱的开发将带来用于高效色谱应用的创新和独特的填充柱。