Exploring Stress Signatures in Marine Fishes

探索海洋鱼类的应激特征

• 批准号: RTI-2020-00653
• 负责人: Easy, Russell
• 金额: $ 3.36万
• 依托单位: Acadia University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=689393
• 关键词: Exploring; Stress; Signatures; Marine; Fishes

Fish diversity and abundance is affected by several factors, including natural stressors such as temperature and predators as well as anthropogenic stressors (e.g. pollution and overfishing). Biomarkers can act as warning signs of physiological disturbances such as loss of homeostasis caused by environmental stressors. My research program explores the identification of biomarkers in response to different types of stressors in fish. These studies are important and timely. When a fish is stressed, bioreactive compounds are released from tissues and organs such as the gill, skin, liver, spleen and kidney. These proteins collect in the mucus of fish and can subsequently be identified using proteomic methods. Stress can also affect the regulation of genes responsible for protein translation, and therefore mechanisms of adaptation can be detected in the DNA and RNA of various fish tissues. It is important to understand how these factors, and their interactions, negatively affect fish health. This novel approach provides hypotheses regarding the metabolic significance of these changes. By examining how these changes are dependent on age, sex and other factors, I will also increase our understanding of the different trade-offs fish may be making to survive in our changing environment. ******I am applying for funding to allow our lab to acquire an imaging system which is essential for our molecular biology and proteomic laboratory. This piece of equipment is an integral part of my new NSERC 2019-2023 research program. I need a dedicated imaging system for visualization of agarose gels in order to identify and quantify nucleic acids resulting from PCR, qPCR and RT-PCR assays. My research program includes testing integrity, quantity and presence of nucleic acids and proteins and an imager is an essential piece of equipment that allows us to do this work. Acadia has integrated modern molecular analyses into may program and our molecular suite spawned numerous publications though we are dependent on a functioning imaging system to continue to produce the high-quality work we are known for. ******The system we are seeking can also be used for chemiluminescence and chemi-fluorescence which are necessary to quantify proteins being used in Western blots. My NSERC funded research program is dependent on such technology and thus this system is essential for me to satisfy my mandate and maintain a modern research facility. Work I am doing in collaboration with Dr. Alejandro Cojen at the Dalhousie University Proteomics facility will be greatly facilitated by this system. Proteins that are separated, visualized and quantified in my lab are further processed using Dr. Cojen's proteomics facility including metabolomics assays and shotgun proteomics techniques. ********

鱼类的多样性和丰富度受到若干因素的影响，包括温度和捕食者等自然压力因素以及人为压力因素（如污染和过度捕捞）。生物标志物可以作为生理紊乱的警告信号，例如由环境压力引起的体内平衡丧失。我的研究项目探索了生物标志物的识别，以应对鱼类不同类型的压力。这些研究是重要和及时的。当鱼受到压力时，生物活性化合物从组织和器官如鳃、皮肤、肝脏、脾脏和肾脏中释放出来。这些蛋白质聚集在鱼的粘液中，随后可以使用蛋白质组学方法进行鉴定。压力也可以影响负责蛋白质翻译的基因的调节，因此可以在各种鱼类组织的DNA和RNA中检测到适应机制。重要的是要了解这些因素及其相互作用如何对鱼类健康产生负面影响。这种新方法提供了关于这些变化的代谢意义的假设。通过研究这些变化如何取决于年龄，性别和其他因素，我也将增加我们对鱼类在不断变化的环境中生存的不同权衡的理解。 ** 我正在申请资金，以使我们的实验室能够获得成像系统，这对我们的分子生物学和蛋白质组学实验室至关重要。这台设备是我新的NSERC 2019-2023研究计划的一个组成部分。我需要一个专用的成像系统，用于琼脂糖凝胶的可视化，以识别和定量PCR、qPCR和RT-PCR检测产生的核酸。我的研究计划包括测试核酸和蛋白质的完整性，数量和存在，成像仪是使我们能够完成这项工作的重要设备。尽管我们依赖于一个功能正常的成像系统来继续生产我们所知道的高质量的工作，但我们已经将现代分子分析整合到了may项目中，我们的分子套件产生了大量的出版物。 ** 我们正在寻找的系统也可以用于化学发光和化学荧光，这是定量蛋白质印迹所必需的。我的NSERC资助的研究计划依赖于这种技术，因此这个系统对我来说是必不可少的，以满足我的任务，并保持一个现代化的研究设施。这个系统将极大地促进我与达尔豪西大学蛋白质组学研究中心的亚历杭德罗·科扬博士合作的工作。在我的实验室中分离、可视化和定量的蛋白质使用Cojen博士的蛋白质组学设施进行进一步处理，包括代谢组学测定和鸟枪蛋白质组学技术。********