Replacement of key laboratory infrastructure for measurement of metabolism in aquatic animals

需要一种Loligo系统光纤、多通道间歇呼吸系统，用于甲壳类动物。该系统由硬件和软件组成，用于测量从细胞水平到大型甲壳类动物的耗氧量（MO2）。这一供资请求还包括一台专门用于该系统的Panasonic防水笔记本电脑。这项资金申请是为了更换McGaw博士实验室的一个关键基础设施。目前，McGaw实验室中有一个较旧（> 10年）的电化学氧气系统，该系统几乎一直由各种受训人员（高素质人员，HQP）使用。然而，它的使用寿命已经结束。该软件不再受支持，系统已多次崩溃。探针本身不断漂移，这使得长期实验不可能，并且该系统只能准确记录较大动物（> 20 g）的MO2。探头本身需要经常注意，并已多次更换。因此，数据的类型和收集准确数据的时间限制了可以执行的许多实验的类型和长度。光纤系统远上级旧的电流系统：它非常精确，传感器不需要维护，几乎零漂移，这使得它适合长期实验。光纤系统可以具有允许“远程”监控的长电缆长度（例如20 m）。此外，计算机、冷水机和水泵会产生影响电流探头的电噪声，系统中使用的Witrox仪器具有与PC的数字无线蓝牙通信功能，因此模拟数据电缆少得多，噪声也小得多。由于传感器具有多种尺寸（<50µm）和快速分辨率，这将使McGaw博士和他的HQP能够准确记录各种不同大小生物体的长期MO2。虽然MO2的测量并不新颖，但收集的数据对于生物生理学实验至关重要，形成了许多论文的主干，然后导致HQP的进一步问题和新项目。该设备将用于培训McGaw实验室现在和未来的研究生，并在不使用时可供其他研究人员使用，因此，将有助于大量受训人员HQP的研究项目和经验。它将成为实验室基础设施的关键部分，将被大量用于各种实验。因此，它也将提供HQP的知识和技能，将在学术环境，政府机构，咨询公司和其他私营部门的公司有价值。

A Loligo systems fiber optic, multi-channel intermittent respiratory system for use in crustaceans is requested. The system consists of hardware and software that is used to measure oxygen consumption (MO2) all the way from the cellular level to that of large crustaceans. This funding request also includes a Panasonic waterproof laptop computer to be dedicated to the system. This funding request is to replace a key piece of infrastructure in Dr. McGaw's lab. There is currently an older (>10yr) galvanic oxygen system in the McGaw lab, which has been in use almost constantly by various trainees (Highly Qualified Personnel, HQP). However, it has come to the end of its useful life. The software is no longer supported and the system has crashed on a number of occasions. The probes themselves drift continually which makes longer term experiments impossible and the system is only accurately able to record MO2 in larger animals (>20g). The probes themselves need constant attention and have been replaced on numerous occasions. Thus the type of data and the time to gather accurate data limits the type and length of a lot of the experiments that can be performed. The fibre optic system is far superior to the older galvanic system: it is highly accurate and the sensors do not need maintenance and have almost zero drift which makes it suitable for longer term experiments. The fibre optic system can have long cable lengths (e.g. 20 m) which allows “remote” monitoring. In addition, computers, water chillers and water pumps generate electrical noise that can affect the galvanic probes, the Witrox instruments used in the system have digital wireless Bluetooth communication with the PC, so there are lot less analog data cables and thus a lot less noise. Because the sensors come in a variety of sizes (<50µm) with rapid resolution it will enable Dr. McGaw and his HQP to accurately record long term MO2 over a wide range of different sized organisms. While the measurement of MO2 is not novel, the data gathered are essential for organismal physiology experiments, forming the backbone of many papers, which then lead on to further questions and new projects for HQP. This equipment will be used to train present and future graduate students in the McGaw lab and will be available for other researchers when not in use, and thus, will contribute to the research projects and experience of a large number of trainees HQP. It will form a key piece of laboratory infrastructure that will be heavily used in a wide variety of experiments. As such it will also provide HQP with the knowledge and skills that will be valuable in the academic environment, to government agencies, and to consulting firms and other private sector companies.