Multimode Microplate Reader with Imaging Capabilities

我们身体中有不同类型的细胞，它们扮演着非常不同的角色，因为它们与全身功能有关。无论是大脑、免疫、肌肉还是循环中的胎儿细胞，了解它们在不同环境中的行为以及它们与身体其他部分的沟通方式将拓宽我们对正常功能和异常功能的可能治疗策略的理解。我们集体研究项目的中心焦点是确定由几种不同的生理模型引起的蛋白质含量、活性和细胞位置的变化。为了更好地单独研究每个细胞或与另一个细胞联合研究，重要的是要有必要的设备来在细胞或亚细胞水平上进行这些测量。在某些情况下，这些测量不仅需要了解细胞内或细胞之间正在发生的事情，而且还需要了解它发生在哪里。*我们希望在我们各自的研究领域使用一种新的仪器--Cytation 5 MV细胞成像多模式微孔板读取器来解决这些问题，该仪器将使我们能够对各种细胞和组织中的RNA和蛋白质含量、蛋白质功能以及抗体的存在进行核心测量。它是市场上这类仪器中最先进的仪器之一，因为它将自动数字成像与传统的多模式微孔板读数相结合，以提供细胞信息和定量数据。它还将让我们看到这些事件在正常情况下发生在哪里，以及在不同的蜂窝环境中出现时。新的仪器还将允许我们检查任何改变细胞功能的环境影响(例如，运动、饮食和药物)。*我们每年在实验室培训2030名高素质人才(HQP)。获得细胞成像微板阅读器是他们接受培训的基础，因为他们将学习只有通过这个系统才能使用的新技术。该仪器的特点将丰富我们的研究成果，允许在同一样本中进行多种测量，并允许HQP更多地接触设备。Cytation 5 MV细胞成像多模式微孔板阅读器对于我们的集体研究计划和及时完成HQP项目是必不可少的。*我们的研究计划采用综合的、自上而下的方法，鼓励HQP在生物、系统、组织、细胞和分子水平上理解生理过程的调节。总而言之，我们的研究为理解代谢性疾病、神经退行性变、炎症、骨骼肌健康和性取向的生物学机制提供了新的方法。

There are different cell types in our body that play very different roles as they relate to whole-body function. Whether it be brain, immune, muscle, or circulating fetal cells, knowing how they behave in different environments and how they communicate with the rest of the body will broaden our understanding of normal function and possible treatment strategies for abnormal function. The central focus of our collective research programs is determining changes in protein content, activity, and cellular location induced by several different physiological models. To better study each cell in isolation or in conjunction with another cell, it is important to have the necessary equipment to make these measurements at cellular or subcellular levels. In some cases, these measurements require an understanding not only of what is happening within a cell or between cells but where it is happening.******We wish to address these questions in our respective research areas with a new instrument, the Cytation 5MV Cell Imaging MultiMode Microplate Reader, that will allow us to make core measures associated with RNA and protein content, protein function, and antibody presence in a variety of cell and tissue types. It is one of the most advanced instruments of this kind on the market as it combines automated digital imaging with conventional multimode microplate reading to provide cellular information and quantitative data. It will also allow us to see where these events are taking place under normal circumstances and when presented with differing cellular environments. The new instrumentation will also allow us to examine any environmental influences (e.g., exercise, diet, and pharmaceuticals) that alter cell function. ******Each year, we train 2030 highly qualified personnel (HQP) in our labs. Access to the cell-imaging microplate reader is foundational to their training in that they will learn new techniques that are only possible with this system. The features of the instrument will enrich our research findings by allowing for a multitude of measures within the same sample, and allow for greater access to equipment by HQP. The Cytation 5MV Cell Imaging MultiMode Microplate Reader is essential for our collective research programs and for the timely completion of HQP projects.******Our research programs take an integrative, top-down approach that encourages HQP to appreciate the regulation of physiological processes at the organism, systems, tissue, cell and molecular levels. Together, our research contributes novel approaches toward understanding the biological mechanisms underpinning metabolic diseases, neurodegeneration, inflammation, skeletal muscle health, and sexual orientation.**