ShEEP Request for a SpectraMax M Series Multi-Mode Microplate Reader

在此应用程序中，我们从ORD共享设备评估计划(SHEEP)申请资金以购买 一种多模式微板阅读器。该仪器将作为共享资源存放在研究处。 奥马哈退伍军人医疗中心的。具体地说，我们寻求获得Spectra Max M系列多模式微型板 读者。SpectrtraMax M系列允许试管和微孔板读取和检测紫外线、可见光、 荧光和发光。这一重要设备将提供最先进的能力，而不是 目前在我们的VAMC园区提供。它将取代我们目前的微板阅读器，它已有10年的历史， 需要广泛而昂贵的维修。有了这种新仪器，就不需要使用昂贵的过滤器来 优化检测级别和背景。光学系统使用两个扫描单色仪，因此 用户可以确定最佳的激发和发射设置，从而实现与 专用单模读卡器。此外，Spectra Max M系列还提供了测量 使用专利的Path Check®传感器，样品的深度(光学路径长度)与温度无关 技术通过SoftMax Pro Software，Path Check传感器可自动使良好的吸光度正常化。 这消除了对标准曲线和具有已知吸收特性的化合物的需要，从而能够 用户可以直接根据吸光度计算浓度。微板阅读器将被广泛应用于 我们在奥马哈VAMC研究服务处的研究人员。该仪器将支持以下新功能 奥马哈VAMC和我们在内布拉斯加大学医学中心(UNMC)的附属机构的研究人员。 收购spectraMax将帮助我们创建该医疗中心目前无法提供的新功能。 SpectraMax M系列将有助于加速我们的研究。具体地说，我们正在审查与退伍军人相关的 临床前疾病模型，包括肝病、糖尿病和肺部疾病，希望推动 将支持退伍军人事务部任务的治疗干预措施。

In this application, we request funds from the ORD Shared Equipment Evaluation Program (ShEEP) to purchase a Multi-Mode Microplate Reader. This instrument will be a shared resource to be housed in the Research Service of the Omaha VA Medical Center. Specifically, we seek to obtain a Spectra Max M Series Multi-Mode Microplate Reader. The SpectraMax M series allows for both cuvette and microplate reading and detection by UV, visible, fluorescence and luminescence. This important piece of equipment will provide state-of-the art capabilities not currently available on our VAMC campus. It will replace our current microplate reader, which is 10 years old and needs extensive and expensive repairs. With this new instrument, there is no need to utilize expensive filters to optimize detection levels and background. The optical systems use two scanning monochromators, so that the user can determine optimal excitation and emission settings, resulting in assay performance similar to that of dedicated single-mode readers. Additionally, the Spectra Max M Series offers the capability of measuring the depth (optical pathlength) of samples with no temperature dependency, using the patented PathCheck® Sensor technology. With SoftMax Pro Software, the PathCheck Sensor automatically normalizes the well absorbance. This eliminates the need for standard curves, and for compounds with known absorbance properties, enabling users to calculate concentrations directly from absorbance. The microplate reader will be extensively used by our investigators in the Research Service of the Omaha VAMC. The instrument will enable new capabilities for researchers at the Omaha VAMC and our affiliates at the University of Nebraska Medical Center (UNMC). Acquisition of the Spectramax will help us create new capabilities not currently available at this Medical Center. SpectraMax M Series will help to accelerate our research. Specifically, we are examining Veteran-relevant, preclinical disease models, including liver disease, diabetes and pulmonary disease in hopes of advancing the therapeutic interventions that will support the VA mission.