Engineering DNAzyme-gold nanoparticle diagnostic device

工程DNAzyme-金纳米颗粒诊断装置

• 批准号: 523436-2018
• 负责人: Chan, Warren
• 金额: $ 17.15万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Collaborative Health Research Projects
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=694649
• 关键词: Engineering; DNAzyme; gold; nanoparticle; diagnostic

Mosquitos carry many infectious pathogens that can significantly affect a patient's health aswell as other people that the patients come into contact with. Currently, there is a major globalscare with the transmission of the Zika virus, which has been implicated in cerebral brainmalformations among fetuses and newborns. Given advances in transportation vehicles,there is a significant risk of the virus being imported back to countries where the mosquitoscarry it are found. There remains an urgent need to develop simple methods to rapidlydiagnose Zika virus infection as it continues to spread to new locations. Rapid diagnosis inareas with reduced access to testing laboratories will allow appropriate management ofpregnant women who become infected and implement control measures as the virus spreadsto new, often remote areas. Here, in this project, our aim is to develop a unique colour-basedtest using nanotechnology components to be able to rapidly diagnose patients that arepresenting symptoms for the Zika virus, as well as two other important mosquito borneviruses, dengue and chikungunya virus. Testing of the 3 viruses is important to determineproper treatment, as patients infected with either of three viruses may present the samesymptoms. We will further develop a smartphone readout system that enables thequantification and monitoring of transmission of Zika virus. A team consisting of an engineerand two medical doctors is assembled to build and test this diagnostic device. Ourcommercial partners have the infrastructure to build the device and reagents, test thediagnostic systems, and to globally sell and distribute the technology for use in patient care.

蚊子携带许多传染性病原体，这些病原体可以显著影响患者的健康以及患者接触的其他人。目前，寨卡病毒的传播引起了全球性的恐慌，该病毒与胎儿和新生儿的大脑畸形有关。鉴于交通工具的进步，寨卡病毒很有可能被输入到蚊子携带病毒的国家。随着寨卡病毒继续传播到新的地方，仍然迫切需要开发简单的方法来快速诊断寨卡病毒感染。在难以进入检测实验室的地区进行快速诊断将有助于对感染的孕妇进行适当管理，并在病毒传播到新的、往往是偏远地区时实施控制措施。在这个项目中，我们的目标是开发一种独特的基于颜色的测试，使用纳米技术组件，能够快速诊断出现寨卡病毒症状的患者，以及其他两种重要的蚊子叮咬病毒，登革热和基孔肯雅病毒。这三种病毒的检测对于确定适当的治疗是很重要的，因为感染三种病毒中任何一种的患者都可能表现出相同的症状。我们将进一步开发智能手机读出系统，以便量化和监测寨卡病毒的传播。一个由一名工程师和两名医生组成的团队被召集起来建造和测试这个诊断设备。我们的商业合作伙伴拥有构建设备和试剂的基础设施，测试诊断系统，并在全球销售和分销用于患者护理的技术。