Understanding pore formation by the complement membrane attack complex

了解补体膜攻击复合物的孔形成

• 批准号: FT150100049
• 负责人: A/Prof Michelle Dunstone
• 金额: $ 55.17万
• 依托单位: Monash University
• 依托单位国家: 澳大利亚
• 项目类别: ARC Future Fellowships
• 财政年份: 2016
• 资助国家: 澳大利亚
• 起止时间: 2016-06-30 至 2021-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/FT150100049
• 关键词: Understanding; pore; formation; complement; membrane

The project aims to improve our understanding of the function of the membrane attack complex (MAC). MAC is a large protein complex used by the human immune system to target invading bacteria and parasites by punching holes in the lipid membranes of target cells. The MAC is part of a superfamily of proteins, the MACPF (membrane attack complex/perforin superfamily)/CDC (cholesterol-dependent cytolysins) superfamily, used by animals (in venoms and immunity), fungi (in defence) and pathogenic bacteria (in disease). The aim of this project is to image to the highest possible resolution how the MAC form pores in the context of bacterial cells and explore the way it inserts into cells in real time. Intended project outcomes may lay the foundation for applied future research into improved antibiotic delivery and novel pesticide development.

该项目旨在提高我们对膜攻击复合物（MAC）功能的理解。MAC是一种大型蛋白质复合物，由人类免疫系统通过在靶细胞的脂质膜上打孔来靶向入侵的细菌和寄生虫。MAC是蛋白质超家族的一部分，MACPF（膜攻击复合物/穿孔素超家族）/CDC（胆固醇依赖性溶细胞素）超家族，被动物（在毒液和免疫中）、真菌（在防御中）和病原菌（在疾病中）使用。该项目的目的是以尽可能高的分辨率成像MAC如何在细菌细胞中形成孔，并探索它真实的插入细胞的方式。预期的项目成果可能为未来改进抗生素输送和新型农药开发的应用研究奠定基础。