Functional Polymers and Capsules

功能聚合物和胶囊

基本信息

  • 批准号:
    RGPIN-2016-06526
  • 负责人:
  • 金额:
    $ 3.35万
  • 依托单位:
  • 依托单位国家:
    加拿大
  • 项目类别:
    Discovery Grants Program - Individual
  • 财政年份:
    2020
  • 资助国家:
    加拿大
  • 起止时间:
    2020-01-01 至 2021-12-31
  • 项目状态:
    已结题

项目摘要

My research involves the development of reactive, multi-responsive polymers and their use in a biomaterials context, including therapeutic cell encapsulation and in cell research. A major focus is on preparing pairs of water-soluble polymers that spontaneously react with each other to form cell-compatible hydrogels. Some of these polymers have cationic and anionic charges that allow them to be coated onto cell-containing calcium alginate capsules, where electrostatic attraction enhances their covalent cross-coupling. The resulting, covalently reinforced cell-containing capsules, are designed for long-term transplantation into, e.g., peritoneal cavities of patients suffering from hormone deficiency disorders such as insulin-dependant Diabetes. These capsules need to balance four requirements: 1. be permeable enough to allow nutrient and insulin diffusion, 2. provide a physical barrier to protect the encapsulated cells from the hosts' immune system, 3. be non-inflammatory themselves, and 4. resist degradation for one year. Current state-of-the-art capsules are largely based on polycation-coated calcium alginate, derived from seaweed, that elicit inflammatory and immune responses due to the polycation, as well as biocontaminants and inflammatory motifs in the alginate. We were able to demonstrate that crosslinked synthetic hydrogel coatings can reduce the hosts' immune response, attributed to both physical shielding of the alginate, and to the strongly hydrophilic nature of these hydrogel coatings due to the presence of excess carboxylate anions. We have since generated a suite of reactive polymers based on polyelectrolytes and polyampholytes with inherently anti-fouling properties. Some of these polymers can form liquid coacervates under physiological conditions, which should enable new modes of deposition onto cell-containing capsules: we will explore depositing liquid coacervates onto calcium alginate capsules, followed by in-situ crosslinking, to form unique micron-range crosslinked shells that may cover the small but critical fraction of cells always protruding from alginate capsules. Some of these polyampholytes have promise as non-cytotoxic cryoprotectants for low temperature storage of stem cells and red blood cells. We further have identified synthetic polymers that may replace alginate in the cell-containing hydrogel core, and will test if they are amenable to crosslinked overcoating, or indeed can be made to spontaneously crosslink once in capsule form. In addition, we have developed giant (10 - 40 micron) monodisperse hydrogel beads formed by precipitation polymerization, that have potential as cell mimics or supports in 3D cell cultures. Our work ranges from fundamental polymer research, to collaboration with stem cell researchers and diabetes researcher/clinicians, aiming to bring synthetic polymers to bear on growing biomedical needs.
我的研究涉及反应性、多响应性聚合物的开发,以及它们在生物材料领域的应用,包括治疗性细胞封装和细胞研究。一个主要的焦点是制备成对的水溶性聚合物,它们可以自发地相互反应,形成与细胞兼容的水凝胶。其中一些聚合物具有阳离子和阴离子电荷,使它们能够被涂覆在含有细胞的海藻酸钙胶囊上,其中静电引力增强了它们的共价交叉偶联。由此产生的共价强化细胞胶囊被设计用于长期移植到患有激素缺乏症患者的腹膜腔内,例如胰岛素依赖型糖尿病患者。这些胶囊需要平衡四个要求:1.足够渗透,以允许营养和胰岛素扩散;2.提供物理屏障,保护被包裹的细胞免受宿主免疫系统的影响;3.本身不发炎;4.在一年内抵抗降解。目前最先进的胶囊主要是基于从海藻中提取的聚阳离子包衣海藻酸钙,由于聚阳离子以及藻酸盐中的生物污染物和炎症基元,它可以引发炎症和免疫反应。我们能够证明,交联型合成水凝胶涂层可以降低宿主的免疫反应,这既归因于海藻酸盐的物理屏蔽,也归因于这些水凝胶涂层由于存在过量的羧酸盐阴离子而具有强烈的亲水性。 自那以后,我们已经开发出了一系列以聚电解质和聚两性聚合物为基础的反应性聚合物,这些聚合物具有固有的防污性能。其中一些聚合物在生理条件下可以形成液体凝聚体,这应该能够在含有细胞的胶囊上形成新的沉积模式:我们将探索将液体凝聚体沉积到海藻酸钙胶囊上,然后进行原位交联,以形成独特的微米级交联壳,这些壳可能覆盖始终从海藻酸钙胶囊中突出的一小部分但关键的细胞。其中一些聚两性离子有望作为无细胞毒性的低温保护剂用于干细胞和红细胞的低温储存。我们进一步确定了可替代细胞水凝胶核心中的藻酸盐的合成聚合物,并将测试它们是否能够进行交联性覆盖,或者确实可以在胶囊形式下自发交联性。此外,我们还开发了通过沉淀聚合形成的巨型(10-40微米)单分散水凝胶微珠,这些微珠具有在3D细胞培养中用作细胞模拟或载体的潜力。我们的工作范围从基础聚合物研究,到与干细胞研究人员和糖尿病研究人员/临床医生的合作,旨在使合成聚合物适应日益增长的生物医学需求。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Stover, Harald其他文献

Stover, Harald的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Stover, Harald', 18)}}的其他基金

Functional Polymers and Colloids
功能聚合物和胶体
  • 批准号:
    RGPIN-2021-04214
  • 财政年份:
    2022
  • 资助金额:
    $ 3.35万
  • 项目类别:
    Discovery Grants Program - Individual
Functional Polymers and Colloids
功能聚合物和胶体
  • 批准号:
    RGPIN-2021-04214
  • 财政年份:
    2021
  • 资助金额:
    $ 3.35万
  • 项目类别:
    Discovery Grants Program - Individual
Functional Polymers and Capsules
功能聚合物和胶囊
  • 批准号:
    RGPIN-2016-06526
  • 财政年份:
    2019
  • 资助金额:
    $ 3.35万
  • 项目类别:
    Discovery Grants Program - Individual
Functional Polymers and Capsules
功能聚合物和胶囊
  • 批准号:
    RGPIN-2016-06526
  • 财政年份:
    2018
  • 资助金额:
    $ 3.35万
  • 项目类别:
    Discovery Grants Program - Individual
Functional Polymers and Capsules
功能聚合物和胶囊
  • 批准号:
    RGPIN-2016-06526
  • 财政年份:
    2017
  • 资助金额:
    $ 3.35万
  • 项目类别:
    Discovery Grants Program - Individual
Functional Polymers and Capsules
功能聚合物和胶囊
  • 批准号:
    RGPIN-2016-06526
  • 财政年份:
    2016
  • 资助金额:
    $ 3.35万
  • 项目类别:
    Discovery Grants Program - Individual
Functional polymers and microspheres
功能聚合物和微球
  • 批准号:
    89661-2006
  • 财政年份:
    2006
  • 资助金额:
    $ 3.35万
  • 项目类别:
    Discovery Grants Program - Individual
Synthesis and study of functional polymers
功能聚合物的合成与研究
  • 批准号:
    89661-2001
  • 财政年份:
    2005
  • 资助金额:
    $ 3.35万
  • 项目类别:
    Discovery Grants Program - Individual
Synthesis and study of functional polymers
功能聚合物的合成与研究
  • 批准号:
    89661-2001
  • 财政年份:
    2004
  • 资助金额:
    $ 3.35万
  • 项目类别:
    Discovery Grants Program - Individual
Synthesis and study of functional polymers
功能聚合物的合成与研究
  • 批准号:
    89661-2001
  • 财政年份:
    2003
  • 资助金额:
    $ 3.35万
  • 项目类别:
    Discovery Grants Program - Individual

相似海外基金

Functional Polymers and Capsules
功能聚合物和胶囊
  • 批准号:
    RGPIN-2016-06526
  • 财政年份:
    2019
  • 资助金额:
    $ 3.35万
  • 项目类别:
    Discovery Grants Program - Individual
Functional Polymers and Capsules
功能聚合物和胶囊
  • 批准号:
    RGPIN-2016-06526
  • 财政年份:
    2018
  • 资助金额:
    $ 3.35万
  • 项目类别:
    Discovery Grants Program - Individual
Functional Polymers and Capsules
功能聚合物和胶囊
  • 批准号:
    RGPIN-2016-06526
  • 财政年份:
    2017
  • 资助金额:
    $ 3.35万
  • 项目类别:
    Discovery Grants Program - Individual
Functional Polymers and Capsules
功能聚合物和胶囊
  • 批准号:
    RGPIN-2016-06526
  • 财政年份:
    2016
  • 资助金额:
    $ 3.35万
  • 项目类别:
    Discovery Grants Program - Individual
Functional polymers and capsules for innovative paper products
用于创新纸制品的功能聚合物和胶囊
  • 批准号:
    250306-2011
  • 财政年份:
    2015
  • 资助金额:
    $ 3.35万
  • 项目类别:
    Discovery Grants Program - Individual
Syntheses and functions of coordination polymers that transform to molecular capsules
转化为分子胶囊的配位聚合物的合成和功能
  • 批准号:
    15K05450
  • 财政年份:
    2015
  • 资助金额:
    $ 3.35万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Functional polymers and capsules for innovative paper products
用于创新纸制品的功能聚合物和胶囊
  • 批准号:
    250306-2011
  • 财政年份:
    2014
  • 资助金额:
    $ 3.35万
  • 项目类别:
    Discovery Grants Program - Individual
Functional polymers and capsules for innovative paper products
用于创新纸制品的功能聚合物和胶囊
  • 批准号:
    250306-2011
  • 财政年份:
    2013
  • 资助金额:
    $ 3.35万
  • 项目类别:
    Discovery Grants Program - Individual
Functional polymers and capsules for innovative paper products
用于创新纸制品的功能聚合物和胶囊
  • 批准号:
    250306-2011
  • 财政年份:
    2012
  • 资助金额:
    $ 3.35万
  • 项目类别:
    Discovery Grants Program - Individual
Functional polymers and capsules for innovative paper products
用于创新纸制品的功能聚合物和胶囊
  • 批准号:
    250306-2011
  • 财政年份:
    2011
  • 资助金额:
    $ 3.35万
  • 项目类别:
    Discovery Grants Program - Individual
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了