Morpho-functional properties of interneurons born at different developmental stages in the adult olfactory bulb

成人嗅球不同发育阶段产生的中间神经元的形态功能特性

• 批准号: RGPIN-2016-05865
• 负责人: Saghatelyan, Armen
• 金额: $ 4.66万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=710005
• 关键词: Morpho; functional; properties; interneurons; born

The olfactory system is one of the oldest sensory modalities and is essential for the survival of many animal species. Olfaction provides vital information about food location and influences social and sexual behaviors. In mammals, the odors are perceived by the olfactory sensory neurons contained within olfactory epithelium. The sensory information is then transferred into the olfactory bulb where it is processed by the bulbar principal cells and interneurons. In the olfactory bulb the population of interneurons largely outnumbers the principal cells by the ratio 100:1 and remarkably, olfactory bulb retains a capacity to renew its neuronal population throughout the life span of animal. The morpho-functional properties of new neurons as well as cells born during early postnatal life remain still elusive. In addition, it is not clear how these cells respond to sensory stimulation and what are their functional and structural modifications in response to odor stimulation. Finally, the role of principall cells in the maturation and integration of cells born at different developmental stages remains completely unclear. We propose the series of experiments aiming at investigation of functional role of neurons born at different developmental periods in the olfactory bulb. By using morphological and electrophysiological recordings, combined with optogenetic stimulation we will test the hypothesis that neurons born in the adulthood bring to the neuronal circuitry properties that cannot be fulfilled by pre-existing population of interneurons. In addition, we will use powerful combination of in vivo intrinsic optical signal (IOS) imaging following odor stimulation, two-photon time-lapse imaging of structural and functional properties of adult-born and early-born interneurons to understand the implications of these cells in the bulbar circuitry. Overall these experiments will allow us to understand why and how the adult brain produces and incorporates new neurons into already existing, functioning circuits of the olfactory bulb.

嗅觉系统是最古老的感觉方式之一，对许多动物物种的生存至关重要。嗅觉提供有关食物位置的重要信息，并影响社会和性行为。在哺乳动物中，气味是由嗅觉上皮内的嗅觉感觉神经元感知的。然后，感觉信息被传输到嗅球，在那里由球主细胞和中间神经元进行处理。在嗅球中，中间神经元的数量远远超过主细胞的数量，比例为100：1，值得注意的是，嗅球在动物的整个生命周期中保持着更新其神经元数量的能力。新生神经元以及出生后早期出生的细胞的形态功能特性仍然难以捉摸。此外，目前还不清楚这些细胞如何对感觉刺激做出反应，以及它们对气味刺激的功能和结构改变是什么。最后，主要细胞在不同发育阶段出生的细胞成熟和整合中的作用仍然完全不清楚。 我们提出了一系列实验，旨在研究不同发育时期出生的神经元在嗅球中的功能作用。通过使用形态和电生理记录，结合光遗传刺激，我们将检验这一假说，即成年期出生的神经元给神经元电路带来的特性是先前存在的中间神经元群体无法满足的。此外，我们将使用气味刺激后的体内固有光信号(IOS)成像和成年和早期出生的中间神经元结构和功能特性的双光子时间推移成像的强大组合，以了解这些细胞在球体电路中的含义。总体而言，这些实验将让我们了解成人大脑为什么以及如何产生新的神经元，并将新神经元整合到已经存在的、正在发挥作用的嗅球回路中。