Temperate Bacteriophages Manipulate Bacterial Populations

温带噬菌体操纵细菌种群

• 批准号: RGPIN-2018-05996
• 负责人: Hynes, Alexander
• 金额: $ 2.7万
• 依托单位: McMaster University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=714025
• 关键词: Temperate; Bacteriophages; Manipulate; Bacterial; Populations

Background: Bacteriophages (phages) are viruses that infect bacteria. They are ubiquitous, abundant, (outnumbering their hosts, bacteria, 10:1), and are estimated to kill 20-40% of all bacteria every day (3)! In a manner akin to predators, they play a critical role in shaping microbial communities (5). By analogy to a more familiar predator's ecological impact, Yellowstone National Park is a healthier and more stable ecosystem since the re-introduction of wolves (6). Phages also exert another very different form of control over bacteria. Some, known as temperate phages, enter a dormant state within the host until conditions favour their awakening (induction). These dormant phages drastically alter the physiology of the bacteria they infect, conferring immunity to other phages (32), encoding new functions (34), and providing some fitness benefits to infected cells (36). Due to ease of discovery as well as their therapeutic potential, there has been a bias towards the study of virulent (non-temperate) phages. Temperate phages are understudied, but far more common, comprising the majority of phages in more stable microbial communities such as that of the human gut (7). Their ability to both kill and directly benefit their hosts make these phages particularly interesting. Vision: Determine the role of temperate phages in shaping stable microbial populations. Approach: Find new phages. I have access to a large collection of previously unculturable gut microbiome isolates. This will serve as the target for isolation of new phages and identification of new signals capable of inducing (awakening) phages from gut bacteria. Assess their impact on their host. Hypothesis: Phage-carrying cells are generally fitter than their phage-free counterparts. By competing infected and uninfected bacteria and observing their phenotypes, I will identify how each new temperate phage is affecting its bacterial host's physiology. Probe phage-containing bacterial communities. Hypothesis: Bacterial communities with temperate phages are more stable. I will create model microbial populations which include phages. This will then allow me to manipulate the environment and the phages in order to observe the effects of those perturbations on the community as a whole. Impact: Phages are master manipulators of bacteria. Isolating new phages is bound to uncover phage-derived tools to manipulate bacteria, much as it has in the past (e.g. anti-CRISPRs, T4 DNA ligase). Furthermore, temperate phages themselves can serve as a non-GMO means to improve the fitness of bacteria used in processes such as bioremediation, biofertilization and bacterial fermentations. Lastly, understanding the role of phages in shaping microbial communities as well as identifying factors that can trigger their phage-driven perturbation/stabilization will aid us in creating stable complex bacterial mixes for biotechnological applications.

背景：噬菌体（Bacteriophages，简称BPHs）是一种感染细菌的病毒。它们无处不在，丰富，（数量超过其宿主，细菌，10：1），估计每天杀死20-40%的细菌（3）！它们以类似于捕食者的方式在塑造微生物群落方面发挥着关键作用（5）。通过类比一个更熟悉的捕食者的生态影响，黄石国家公园是一个更健康，更稳定的生态系统，因为狼的重新引入（6）。噬菌体对细菌的控制还有另一种非常不同的形式。一些被称为温和的寄生虫，在宿主体内进入休眠状态，直到条件有利于它们的觉醒（诱导）。这些休眠的细菌极大地改变了它们感染的细菌的生理机能，赋予对其他细菌的免疫力（32），编码新的功能（34），并为感染的细胞提供一些健康益处（36）。 由于易于发现以及它们的治疗潜力，一直存在对毒性（非温和）真菌的研究的偏见。温带细菌研究不足，但更常见，包括更稳定的微生物群落中的大多数细菌，如人类肠道（7）。它们既能杀死宿主，又能直接使宿主受益的能力，使这些寄生虫特别有趣。 愿景：确定温带气候在形成稳定微生物种群中的作用。 方法： 找到新的目标。我可以接触到大量以前无法培养的肠道微生物组分离物。这将作为分离新细菌和鉴定能够从肠道细菌诱导（唤醒）细菌的新信号的靶标。 评估它们对宿主的影响。假设：携带噬菌体的细胞通常比不携带噬菌体的细胞更健康。通过竞争感染和未感染的细菌，并观察它们的表型，我将确定每一个新的温和噬菌体是如何影响其细菌宿主的生理。 探测含噬菌体的细菌群落。假设：温带的细菌群落更稳定。我将创建包括细菌在内的微生物种群模型。这将使我能够操纵环境和环境，以便观察这些扰动对整个社区的影响。 影响：噬菌体是细菌的主要操纵者。分离新的噬菌体势必会发现噬菌体衍生的工具来操纵细菌，就像过去一样（例如抗CRISPR，T4 DNA连接酶）。此外，温和的微生物本身可以作为一种非转基因手段，以改善生物修复，生物施肥和细菌发酵等过程中使用的细菌的适应性。最后，了解细菌在塑造微生物群落中的作用，以及确定可以触发其噬菌体驱动的扰动/稳定的因素，将有助于我们为生物技术应用创造稳定的复杂细菌混合物。