Decoding the mammalian regulatory genome

解码哺乳动物调控基因组

• 批准号: RGPIN-2020-05972
• 负责人: Mitchell, Jennifer
• 金额: $ 5.03万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=718134
• 关键词: Decoding; mammalian; regulatory; genome

Despite completion of the human genome sequence 16 years ago we are currently not able to determine the function of the vast majority of this sequence. We understand how to read the sequence code within gene coding regions but these are We will address three key questions: 1) Why are so many transcription factor binding sites needed for enhancer activity? 2) How is the regulatory code different in different cell types? 3) What is the best computational approach to fully decode mammalian genomes? In the long term fully decoding genome sequences will allow us to predict how regulatory sequence changes will affect the expression of specific genes. This will have widespread impact on our understanding of development, evolution and the underlying cause of phenotype variation in populations.

尽管人类基因组序列在16年前就完成了，但我们目前还无法确定该序列中绝大多数的功能。我们了解如何读取基因编码区内的序列密码，但以下是我们将解决的三个关键问题： 1)为什么增强子活性需要这么多转录因子结合位点？ 2)不同细胞类型的监管代码有何不同？ 3)完全破译哺乳动物基因组的最佳计算方法是什么？ 从长远来看，完全解码基因组序列将使我们能够预测调控序列的变化将如何影响特定基因的表达。这将对我们理解种群的发育、进化和表型变异的根本原因产生广泛的影响。