Multiflow system for stable isotope analyses

用于稳定同位素分析的多流系统

• 批准号: RTI-2021-00562
• 负责人: deVernal, Anne
• 金额: $ 9.06万
• 依托单位: Université du Québec à Montréal
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=718846
• 关键词: Multiflow; system; stable; isotope; analyses

The stable isotopes of carbon and oxygen in liquid, gas and solid compounds are tracers of biological and geochemical processes that are used for investigations in many disciplinary fields. The equipment (MultiFlow Geo) requested here is a gas extraction system to be coupled with an isotopic ratio mass spectrometer for the separation of CO2 from other gases and its introduction for measurements of low abundance carbon and oxygen isotopes. The equipment includes an autosampler that enables high throughput and unattended continuous analyses. It also includes an automated acid digestion unit for the recovery of CO2 from carbonates. The instrument optimizes operations and reduces measurement uncertainty compared to the current manually operated reaction vessels, allowing for sample sizes from less than 10 micrograms to milligrams and, thus, measurements of single shells of foraminifer (unicellular microorganism) as well as seasonal growth layers of teeth or stalagmites. The autosampler with temperature-controlled tray and gas separation system can process one sample every 3 minutes. Complementary kits will allow analyses of 1) hydrogen and oxygen isotopes in water for hydrology investigations, 2) carbon isotopes of dissolved inorganic carbon for studies of the carbon cycle, and 3) carbon and oxygen isotopes in breath CO2, for biomedical applications. The requested instrument will replace the current introduction system (MicroGas), acquired 22 years ago, that has generated more than 23 000 analyses over the last 15 years, for 25 university professors across Canada and 40 of their students. This old system cannot be configured to automatically inject acid nor flush reaction vials, nor run micro-samples. The proposed instrument would permit a large array of new experiments and research projects in addition to significantly enhancing the current throughput. It will complement the world-class light stable isotopes infrastructure of the Geotop research center in Earth system science, which has contributed to the training of more than 130 students, adding analytical support for the private sector. The main research projects of the applicant and co-applicants to be conducted with the instrument focus on a) the climate history and variations of sea ice cover in the Arctic Ocean, b) long-term ice-ocean-atmosphere interactions regulating the Earth climate, c) the seasonal climate contrasts and extremes in geological archives such as teeth of fossil mammals and cave stalagmites, d) the reconstruction of diets of prehistorical hominids in relation with climate, e) ocean acidification, and f) the renewal and quality of groundwater resources. In addition to the co-applicants of the present request, the equipment will be used by a large community of users from the private and academic sectors. On a yearly basis, about 20 students, several postdoctoral and visiting scholars will be trained for stable isotope measurements using the gas extraction system.

液体，气体和固体化合物中碳和氧的稳定同位素是生物学和地球化学过程的示踪剂，用于在许多学科领域进行研究。此处要求的设备（多量GEO）是一种气体提取系统，可与同位素比质谱仪耦合，以将CO2与其他气体分离，并引入了低丰度碳和氧气同位素的测量。该设备包括一个自动采样器，可实现高吞吐量和无人看管的连续分析。它还包括一个自动酸消化单元，用于从碳酸盐中回收二氧化碳。与当前手动反应容器相比，该仪器优化了操作并减少了测量不确定性，从而使样本量从少于10微克到毫克，从而使样本量从少数微克到毫克，从而可以测量有孔虫的单个壳（单细胞微生物）的单个壳，以及牙齿或牙齿的季节生长层。具有温度控制的托盘和气体分离系统的自动采样器每3分钟可以处理一个样本。互补试剂盒将允许对水中的水中的氢和氧同位素进行分析，以进行水文研究，2）溶解的无机碳的碳同位素进行碳循环研究，以及3）呼吸二氧化碳中的碳和氧同位素，用于生物代理应用。 该请求的仪器将替换22年前收购的当前引入系统（Microgas），该系统在过去15年中已为25年的25个大学教授和40名学生提供了超过23,000个分析。该旧系统不能配置为自动注入酸或冲洗反应小瓶，也不能运行微型样本。该拟议的仪器将允许大量的新实验和研究项目，除了显着增强当前的吞吐量。它将补充地球系统科学地理研究中心的世界一流的光稳定同位素基础设施，该基础设施为培训130多名学生提供了贡献，为私营部门增加了分析支持。 The main research projects of the applicant and co-applicants to be conducted with the instrument focus on a) the climate history and variations of sea ice cover in the Arctic Ocean, b) long-term ice-ocean-atmosphere interactions regulating the Earth climate, c) the seasonal climate contrasts and extremes in geological archives such as teeth of fossil mammals and cave stalagmites, d) the reconstruction of diets of与气候，e）海洋酸化和f）地下水资源的更新和质量有关的史前人类术。除了本请求的共同申请人外，该设备还将由私人和学术领域的大型用户社区使用。 每年，大约有20名学生，将使用气体提取系统对稳定的同位素测量进行培训。