Acquisition of a focused-ultrasonicator system

购置聚焦超声仪系统

• 批准号: RTI-2021-00702
• 负责人: Gévry, Nicolas
• 金额: $ 9.04万
• 依托单位: Université de Sherbrooke
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=718871
• 关键词: Acquisition; focused; ultrasonicator; system

For a number of years, the NSERCfunded research programs of at least 11 PIs of the Université de Sherbrooke Biology department and their > 80 trainees and staff scientists have been hampered by the limitations of our probe and bath sonicators for their molecular biology, genomics and system biology approaches. Focused-ultrasonicators with Adaptive Focused Acoustics (AFA) are a powerful technology that uses acoustic energy to mechanically process samples. Contrary to traditional sonication systems, the AFA technology employs focused bursts of ultrasonic acoustic energy in a highly controllable and precise isothermal process. As a consequence, experimental protocols are significantly easier to standardize, results are highly reproducible, and more challenging methods can be implemented using this innovative approach. Ultrasonicators offering the AFA technology can be used in a wide variety of applications and are the method of choice for the mechanical fragmentation of nucleic acids for Next-Generation-Sequencing (NGS). Our research teams use NGS technologies as an everyday investigative tool to address questions in synthetic biology, cancer, reproduction, metabolism, host-microbe interactions, among others. Moreover, AFA technology is an extremely versatile and highly controllable technique that not only standardizes the sample preparation for NGS, but also allows nuclear isolation, biofilm disruption, and drug solubilization as well as a number of other methods. The requested ML230 focused-ultrasonicator will allow methodological advances that will help us to rapidly increase productivity and that will enable new cutting-edge applications. We request funding to purchase this instrument that is otherwise unavailable at the Université de Sherbrooke. Acquisition of this system is essential for our research programs and will enable many new projects as well as contribute in a major way to the training of highly qualified personnel from several laboratories in the Biology department and the Faculty of Sciences of our University.

多年来，nserc资助的舍布鲁克大学生物系的至少11个pi和他们的bb80名学员和工作人员的研究项目，由于我们的探针和浴声器在分子生物学、基因组学和系统生物学方法上的局限性而受到阻碍。