Acquisition of an in vivo imaging system

自第一台显微镜问世以来，获得细胞、组织和整个动物图像的能力一直处于生物技术进步的前沿，并导致了各种成像方式的发展，包括光学成像。生物发光和近红外（NIR）荧光光学成像允许各种细胞过程的可视化，并且已经从从单个时间点观察组织之间的解剖差异发展到以无创方式纵向成像同一动物或植物（幼苗）的多种生物特征。直到最近，体内成像需要非常昂贵（通常是25万美元）的仪器，而且操作起来相对复杂。相比之下，新要求的珍珠三部曲体内成像系统价格合理，操作简单。这种生物发光和近红外体内成像系统提供了足够的目标成像选择，以满足绝大多数（>95%）的实验需求。我们请求资助购买这台仪器，否则在舍布鲁克大学主校区是不可用的，以支持我们在许多领域使用小动物和植物体内成像的研究。该仪器对我们的研究项目至关重要，并且将在成像平台协调员的监督下，使许多新项目成为可能，并有助于培训来自多个实验室的高素质人员。

The ability to obtain images of cells, tissues, and whole animals has been at the forefront of biological technological advance since the advent of the first microscope and has resulted in the evolution of various imaging modalities, including optical imaging. Bioluminescent and near-infrared (NIR) fluorescent optical imaging allows the visualization of various cellular processes and has evolved from observing anatomical differences between tissues from a single time point to imaging multiple biological features longitudinally in a noninvasive manner in the same animal or plant (seedling). Until recently, in vivo imaging required very expensive (often >$250,000) instruments that were also relatively complex to operate. In contrast, the requested new Pearl Trilogy in vivo imaging system is affordable and simple to run. This bioluminescent and NIR in vivo imaging system delivers enough targets imaging options for the vast majority (>95%) of experimental needs in our laboratories. We request funding to purchase this instrument, that is otherwise unavailable on the main campus of the Université de Sherbrooke, to support our research in many fields using small animals and plants in vivo imaging. This instrument is essential for our research programs, and will, under the supervision of a imaging platform coordinator, enable many new projects as well as contribute to the training of highly qualified personnel from several laboratories.