Acquisition of an in vivo imaging system

获取体内成像系统

• 批准号: RTI-2017-00350
• 负责人: Gévry, Nicolas
• 金额: $ 10.55万
• 依托单位: Université de Sherbrooke
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2016
• 资助国家: 加拿大
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=616648
• 关键词: Acquisition; vivo; imaging; system

The ability to obtain images of cells, tissues, and whole animals has been at the forefront of biological technological advance since the advent of the first microscope and has resulted in the evolution of various imaging modalities, including optical imaging. Bioluminescent and near-infrared (NIR) fluorescent optical imaging allows the visualization of various cellular processes and has evolved from observing anatomical differences between tissues from a single time point to imaging multiple biological features longitudinally in a noninvasive manner in the same animal or plant (seedling). Until recently, in vivo imaging required very expensive (often >$250,000) instruments that were also relatively complex to operate. In contrast, the requested new Pearl Trilogy in vivo imaging system is affordable and simple to run. This bioluminescent and NIR in vivo imaging system delivers enough targets imaging options for the vast majority (>95%) of experimental needs in our laboratories. We request funding to purchase this instrument, that is otherwise unavailable on the main campus of the Université de Sherbrooke, to support our research in many fields using small animals and plants in vivo imaging. This instrument is essential for our research programs, and will, under the supervision of a imaging platform coordinator, enable many new projects as well as contribute to the training of highly qualified personnel from several laboratories.

自第一台显微镜问世以来，获得细胞、组织和整个动物图像的能力一直处于生物技术进步的最前沿，并导致了包括光学成像在内的各种成像方式的发展。生物发光和近红外（NIR）荧光光学成像允许各种细胞过程的可视化，并且已经从单个时间点观察组织之间的解剖差异发展到以非侵入性方式在同一动物或植物（幼苗）中纵向成像多个生物特征。直到最近，体内成像需要非常昂贵（通常> 250，000美元）的仪器，这些仪器操作起来也相对复杂。相比之下，所要求的新型Pearl Trilogy体内成像系统价格实惠，运行简单。这种生物发光和近红外活体成像系统为我们实验室的绝大多数（>95%）实验需求提供了足够的目标成像选项。我们请求资助购买这种仪器，否则在舍布鲁克大学的主校区无法获得，以支持我们在许多领域使用小动物和植物体内成像的研究。该仪器对我们的研究计划至关重要，并将在成像平台协调员的监督下，使许多新项目成为可能，并有助于培训来自多个实验室的高素质人员。