Physical Properties and Colloidal Stability of Aluminium Hydroxide Adjuvants (RehydragelTM)

氢氧化铝佐剂 (ReHydragelTM) 的物理性质和胶体稳定性

• 批准号: 570656-2021
• 负责人: Tam, Michael
• 金额: $ 2.91万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2021
• 资助国家: 加拿大
• 起止时间: 2021-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=722056
• 关键词: Physical; Properties; Colloidal; Stability; Aluminium

Chemtrade produces an extensive line of highly versatile aluminum-based Vaccine Adjuvants (AVA) that can be used over a broad range of pH, types of protein binding, and other vaccine requirements. The control of the processing parameters in the production of AVA control their physical properties, such as stability, rheology, particle size, which have an impact on their efficacy as vaccine adjuvants. The proposed research will focus on elucidating the relationship between the surface characteristics of AVA on their stability and efficacy as vaccine adjuvants. The key properties that will be quantified are, (a) particle size, (b) zeta potential, (c) morphology, (d) rheology and (e) surface composition of the nanoparticles. We will develop synthetic protocols to elucidate the parameters that control the colloidal stability of the Rehydragel adjuvants. Additionally, we will conduct QA analyses of the Rehydragel samples from production batches in order to elucidate the relationship between processing parameters and the physical properties of the AVA. The key objective of the project is to develop fundamental understanding on the colloidal chemistry of the AVA, and to use this knowledge to advance and improve the performance of the AVA, in terms of their long-term stability and their efficacy as vaccine adjuvants. Additionally, we will train highly qualified personnels to support the development and production of better AVA to support the growing demands and needs of vaccine in human and veterinary applications.

Chemtrade生产一系列高度通用的铝基疫苗佐剂（AVA），可用于广泛的pH值范围、蛋白质结合类型和其他疫苗要求。在AVA的生产过程中，工艺参数的控制控制了其物理性质，如稳定性、流变性、粒度等，这些都对其作为疫苗佐剂的功效有影响。拟开展的研究将集中于阐明AVA的表面特性与其作为疫苗佐剂的稳定性和效力之间的关系。将被量化的关键性质是(a)颗粒大小，(b) zeta电位，(c)形貌，(d)流变性和(e)纳米颗粒的表面组成。我们将制定合成方案，以阐明控制Rehydragel佐剂胶体稳定性的参数。此外，我们将对生产批次的Rehydragel样品进行QA分析，以阐明工艺参数与AVA物理性质之间的关系。该项目的主要目标是发展对AVA胶体化学的基本理解，并利用这些知识来推进和改善AVA的性能，就其长期稳定性和作为疫苗佐剂的功效而言。此外，我们将培训高素质的人员，以支持开发和生产更好的AVA，以支持人类和兽医应用中对疫苗日益增长的需求。