2-ZYME: Two-step biocatalytic conversion of underused biorefinery side-streams to glucaric acid

2-ZYME：将未充分利用的生物精炼厂副产物两步生物催化转化为葡萄糖二酸

• 批准号: 560573-2021
• 负责人: Master, Emma
• 金额: $ 9.11万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Idea to Innovation
• 财政年份: 2021
• 资助国家: 加拿大
• 起止时间: 2021-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=723910
• 关键词: ZYME; Two; step; biocatalytic; conversion

The 2-ZYME technology applies the selectivity of bio-catalytic conversions to transform underused biomass fractions of major bioresource sectors into sought-after biochemicals. By leveraging natural structures in underused biomass, the 2-ZYME technology increases bioresource efficiency while diversifying the product portfolio of existing biorefineries.The technology combines two enzymes in one pot to produce glucaric acid from glucuronoxylans an underused fraction of wood and agricultural biorefineries making cellulose pulps and bioethanol, respectively. Glucaric acid is a platform chemical that serves as a key building block for biopolymer synthesis, and is also used as such in phosphate-free detergents along with de-icing agents. The global glucaric acid market size was estimated at USD 550.4 million in 2016 with a projected cumulative average growth rate (CAGR) of 7% during the 2018-2023 period. Advantages of the 2-ZYME technology over the conventional approach to producing glucaric acid include high product purity and yield, and low feedstock costs.A recently completed NSERC I2I Market Assessment of the 2-ZYME technology confirmed the market readiness for this new enzyme system. The current NSERC I2I Phase I project will focus on three specific tasks needed to secure private sector investment: 1) detailed compositional analysis of underused biomass streams produced by existing biorefineries to inform deployment efforts, 2) application of the 2-ZYME technology to the prioritized glucuronoxylan sources, and 3) prove out lab scale-up of the 2-ZYME technology including product recovery.To achieve the project goals, the proposed action assembles committed industrial partners, including feedstock providers (Fortress Advanced Bioproducts, IGPC Ethanol), biotechnology developers (Novozymes), and market experts (Pansophy Consulting), to create a focused academic-industry partnership that is motivated and fully equipped to realize the economic potential and environmental benefits of the 2-ZYME technology.

2-ZYME 技术应用生物催化转化的选择性，将主要生物资源部门未充分利用的生物质部分转化为受欢迎的生物化学品。通过利用未充分利用的生物质中的天然结构，2-ZYME 技术提高了生物资源效率，同时使现有生物精炼厂的产品组合多样化。该技术将两种酶结合在一锅中，从葡萄糖醛酸木聚糖（木材中未充分利用的部分）生产葡萄糖二酸，并分别用于生产纤维素纸浆和生物乙醇的农业生物精炼厂。葡萄糖二酸是一种平台化学品，是生物聚合物合成的关键组成部分，也可与除冰剂一起用于无磷酸盐洗涤剂。 2016年全球葡萄糖酸市场规模估计为5.504亿美元，预计2018-2023年期间累计平均增长率（CAGR）为7%。与生产葡萄糖酸的传统方法相比，2-ZYME 技术的优势包括产品纯度和产量高，以及原料成本低。最近完成的 NSERC I2I 2-ZYME 技术市场评估证实了这种新酶系统的市场准备情况。当前的 NSERC I2I 第一阶段项目将重点关注确保私营部门投资所需的三项具体任务：1）对现有生物炼制厂生产的未充分利用的生物质流进行详细成分分析，为部署工作提供信息；2）将 2-ZYME 技术应用于优先的葡糖醛酸木聚糖来源；3）证明 2-ZYME 技术的实验室规模扩大，包括产品回收。为了实现项目目标，拟议的行动包括 包括原料供应商（Fortress Advanced Bioproducts、IGPC Ethanol）、生物技术开发商（Novozymes）和市场专家（Pansophy Consulting）在内的忠诚的工业合作伙伴，建立一个专注的学术-工业合作伙伴关系，该合作伙伴关系有动力且完全有能力实现 2-ZYME 技术的经济潜力和环境效益。