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A simple and cost-effective test for water-borne pathogen Legionella

一种简单且经济有效的水传播病原体军团菌测试

基本信息

批准号：
561562-2021
负责人：
Li, Yingfu
金额：
$ 8.96万
依托单位：
McMaster University
依托单位国家：
加拿大
项目类别：
Idea to Innovation
财政年份：
2021
资助国家：
加拿大
起止时间：
2021-01-01 至 2022-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=724261
关键词：
simple cost effective test water

项目摘要

Legionella pneumophila (Legionella) is a waterborne pathogen from naturally occurring and man-made water sources and is known to cause Legionnaires' disease. In recent years, Legionella outbreaks continue to arise both in Canada and around the world. One in 10 people in the general public and one in 4 people from health care facilities contracted with Legionella will die. Therefore the economic and human costs of Legionella outbreaks are very high. There is currently a lack of cost-effective and simple diagnostic testing and monitoring methods for Legionella pneumophila. Synthetic DNA-based enzymes (DNAzymes) are capable of catalyzing a wide variety of chemical reactions and we believe are well suited to address the need for fast and simple Legionella testing methods by acting as a molecular recognition element. Our team has already developed an RNA-cleaving DNAzyme that demonstrates highly selective RNA-cleaving activity towards Legionella. The key objectives of this project are to optimize the performance of this DNAzyme for detecting Legionella and to create a simple biosensor prototype for commercialization. When developed, this biosensor holds the potential to provide a simple and user-friendly solution for on-site monitoring of the contamination of Legionella in exposure sources, such as cooling water towers.

嗜肺军团菌（Legionella pneumophila，简称Legionella）是一种来自天然和人造水源的水媒病原体，已知可引起军团病。近年来，军团菌疫情继续在加拿大和世界各地出现。一般公众中每10人中就有一人和卫生保健设施中每4人中就有一人感染军团菌会死亡。因此，军团菌爆发的经济和人力成本非常高。目前缺乏具有成本效益和简单的嗜肺军团菌诊断检测和监测方法。基于DNA的合成酶（DNAzymes）能够催化各种化学反应，我们认为，通过充当分子识别元件，它非常适合于满足快速和简单的军团菌检测方法的需求。我们的团队已经开发出一种RNA切割DNAzyme，它对军团菌表现出高度选择性的RNA切割活性。该项目的主要目标是优化该DNAzyme用于检测军团菌的性能，并创建用于商业化的简单生物传感器原型。当开发时，这种生物传感器有可能提供一个简单和用户友好的解决方案，用于现场监测暴露源（如冷却水塔）中的军团菌污染。